Multitasking in replication is common among geminiviruses
Multitasking in replication is common among geminiviruses
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DOI：
10.1128/jvi.77.5.2972-2980.2003
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发表时间：
2003-03-01
影响因子：
5.4
通讯作者：
Jeske, H
中科院分区：
文献类型：
--
作者：
Preiss, W;Jeske, H
双生病毒包装单链环状DNA并通过双链DNA中间体复制。在过去的十年中，越来越多的证据使人们普遍接受它们的复制遵循滚环复制机制，就像具有单链DNA的噬菌体一样。在最近的一项研究中，我们表明，这也是真实的Abutilon花叶双生病毒（AbMV），但这种特殊的病毒也可以使用重组依赖性复制（RDR）的途径类似于T4病毒。由于AbMV是一个特例，因为它已经在观赏植物上繁殖了一百多年，所以确定RDR在其他双生病毒中是否常见是有趣的。我们分析了双生病毒从不同的属和地理起源，使用BND纤维素色谱结合改进的高分辨率二维凝胶电泳，我们得出结论，多任务复制是普遍的，至少对于非洲木薯花叶，甜菜曲顶，番茄金色花叶，番茄黄化曲叶病毒。
Geminiviruses package single-stranded circular DNA and replicate via double-stranded DNA intermediates. During the past decade, increasing evidence has led to the general acceptance that their replication follows a rolling-circle replication mechanism like bacteriophages with single-stranded DNA. In a recent study, we showed that this is also true for Abutilon mosaic geminivirus (AbMV), but that this particular virus may also use a recombination-dependent replication (RDR) route in analogy to T4 phages. Because AbMV is a special case, since it has been propagated on ornamental plants for more than a hundred years, it was interesting to determine whether RDR is common among other geminiviruses. We analyzed geminiviruses from different genera and geographic origins by using BND cellulose chromatography in combination with an improved high resolution two-dimensional gel electrophoresis, and we conclude that multitasking in replication is widespread, at least for African cassava mosaic, Beet curly top, Tomato golden mosaic, and Tomato yellow leaf curl virus.