An exercise degree monitoring biosensor based on electrochemiluminescent detection of lactate in sweat
An exercise degree monitoring biosensor based on electrochemiluminescent detection of lactate in sweat
复制标题
基于电化学发光检测汗液中乳酸的运动程度监测生物传感器
DOI：
10.1016/j.snb.2009.10.002
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发表时间：
2010-01-07
影响因子：
8.4
通讯作者：
Tu, Yifeng
中科院分区：
文献类型：
--
作者：
Cai, Xia;Yan, Jilin;Tu, Yifeng
以鲁米诺作为信号物质开发了一种基于电化学发光 (ECL) 的乳酸 (LA) 生物传感器。 LA在固定化乳酸脱氢酶(LDH)和丙酮酸氧化酶(PYOD)的催化下，以烟酰胺腺嘌呤二核苷酸(NAD)为辅酶被氧化。结果，反应产生了过氧化氢，增强了鲁米诺的电化学发光，这对于感测 LA 浓度是可以接受的。由此产生的生物传感器对乳酸表现出优异的响应。真实汗液样品的检出限为8.9×10(-12)mol/L，相对标准偏差(RSD)为4.13%(C-LA 1.34×10(-10)mol/L，n=6)，平均回收率为101.3%。研究表明，基于ECL的生物传感器可用于检测运动员训练期间汗液样本中的LA，以评估运动程度。 (C) 2009 Elsevier B.V. 保留所有权利。
An electrochemiluminescent (ECL)-based lactate (LA) biosensor is developed with luminol as signaling species. LA was oxidized under the catalysis of immobilized lactic dehydrogenase (LDH) and pyruvate oxidse (PYOD) with nicotinamide adenine dinucleotide (NAD) as coenzyme. As a result, the reaction yielded the hydrogen peroxide, which enhanced the electrochemiluminescence of luminol, which was acceptable for sensing the LA concentration. The resulting biosensor showed excellent response for lactate. A detection limit of 8.9 x 10(-12) mol/L was obtained with the relative standard deviation (RSD) of 4.13% (C-LA 1.34 x 10(-10) mol/L, n = 6) and an average recovery of 101.3% for real sweat sample. The research suggests that the ECL-based biosensor was practicable for detecting the LA in sweat samples of athletes during the exercitation for evaluation of exercise degree. (C) 2009 Elsevier B.V. All rights reserved.