The tertiary structure of the four-way DNA junction affords protection against DNase I cleavage.
The tertiary structure of the four-way DNA junction affords protection against DNase I cleavage.
复制标题
四路 DNA 连接的三级结构可提供针对 DNase I 切割的保护。
DOI：
10.1093/nar/18.9.2599
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发表时间：
1990
影响因子：
14.9
通讯作者：
D. Lilley
中科院分区：
文献类型：
--
作者：
A. Murchie;W. Carter;J. Portugal;D. Lilley
用核酸酶DNase I探测了四向螺旋连接DNA中磷酸二酯键的可及性。在所有四股接头上观察到保护区域，在同轴堆叠的螺旋对之间交换的股上往往更长。堆叠螺旋的连续链上的受保护区域并不精确地位于连接处，而是朝向链的3'侧移位。这是位于非交叉的右手结构中相对螺旋的大沟中的骨架区域，因此可以预测其被保护免受酶的切割。然而，结构的主要凹槽仍然可以被小得多的探针硫酸二甲酯接近。
The accessibility of phosphodiester bonds in the DNA of four-way helical junctions has been probed with the nuclease DNase I. Regions of protection were observed on all four strands of the junctions, that tended to be longer on the strands that are exchanged between the coaxially stacked pairs of helices. The protected regions on the continuous strands of the stacked helices were not located exactly at the junction, but were displaced towards the 3' side of the strand. This is the region of backbone that becomes located in the major groove of the opposed helix in the non-crossed, right-handed structure for the junction, and might therefore be predicted to be protected against cleavage by an enzyme. However, the major grooves of the structure remain accessible to the much smaller probe dimethyl sulphate.