Three-dimensional digital microscopy using the PHOIBOSl Scanner
Three-dimensional digital microscopy using the PHOIBOSl Scanner
复制标题
使用 PHOIBOSl 扫描仪的三维数字显微镜
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
S. Wahlsten
中科院分区：
文献类型：
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作者：
N. Aslund;A. Liljeborg;P. Forsgren;S. Wahlsten
通过将共聚焦扫描与数字记录和图像处理相结合，开辟了荧光显微镜的新视角。使用通过共焦扫描获得的一系列连续光学切片来生成构成数字三维显微镜的基础的数据体。介绍了一个基于这些原理的仪器系统PHOIBOS，并给出了该系统所取得的一些有代表性的成果。与传统方法(例如，使用切片机)相比，一个很大的优点是标本不会损坏。不会像机械切片时那样引入任何瑕疵。该方法还可用于研究荧光光或反射光中的表面拓扑结构。
New perspectives are opened in fluorescence microscopy by combining confocal scanning with digital recording and image processing. A series of consecutive optical sections obtained by confocal scanning are used to generate a data volume that constitutes a basis for digital three-dimensional microscopy. A description is given of an instrument system, PHOIBOS, which works according to these principles, and some representative results achieved with the system are presented. A great advantage as compared with traditional methods (e.g., using a microtome) is that the specimen is left undamaged. No artifacts are introduced as when slicing mechanically. The method can also be used to study surface topologies either in fluorescent or reflected light.