Quantitative isolation of biocontrol agents Trichoderma spp., Gliocladium spp. and actinomycetes from soil with culture media
Quantitative isolation of biocontrol agents Trichoderma spp., Gliocladium spp. and actinomycetes from soil with culture media
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DOI：
10.1016/j.micres.2006.11.022
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发表时间：
2009-01-01
影响因子：
6.7
通讯作者：
March, G. J.
中科院分区：
文献类型：
--
作者：
Gil, S. Vargas;Pastor, S.;March, G. J.
土壤生物多样性在农业系统的可持续性中发挥着关键作用，并表明土壤的健康水平，特别是在考虑到涉及生物的微生物的丰富性时。土传病害的防治。栽培措施可能会引起土壤微生物区系的变化，这可以通过分离目标微生物来量化。根际土壤样品取自不同轮作和耕作制度下的土壤样品，以及木霉属(Trichoderma spp。并对放线菌进行了定量化，以选择能更好地反映土壤中这些微生物种群变化的通用和选择性培养基组分。木霉菌分离最有效的培养基为。和粘杆菌属Gliocladium spp.马铃薯葡萄糖琼脂添加氯霉素、链霉素和孟加拉玫瑰，放线菌采用Kuster培养基，添加环己亚胺和丙酸钠。(C)2007年爱思唯尔股份有限公司。版权所有。
Soil biodiversity plays a key role in the sustainability of agriculture systems and indicates the level of health of soil, especially when considering the richness of microorganisms that are involved in biological. control of soilborne diseases. Cultural practices may produce changes in soil microflora, which can be quantified through the isolation of target microorganisms. Rhizosphere soil samples were taken from an assay with different crop rotations and tillage systems, and populations of Trichoderma spp., Gliocladium spp. and actinomycetes were quantified in order to select the general and selective culture media that better reflect the changes of these microbial populations in soil. The most efficient medium for the isolation of Trichoderma spp. and Gliocladium spp. was potato dextrose agar modified by the addition of chloramphenicol, streptomycin and rose bengal, and for actinomycetes was Kuster medium, with cycloheximide and sodium propionate. (C) 2007 Elsevier GmbH. All rights reserved.