Co-spread of metal and antibiotic resistance within ST3-IncHI2 plasmids from E. coli isolates of food-producing animals.
Co-spread of metal and antibiotic resistance within ST3-IncHI2 plasmids from E. coli isolates of food-producing animals.
复制标题
来自食品生产动物的大肠杆菌分离株的 ST3-IncHI2 质粒中金属和抗生素耐药性的共同传播。
DOI：
10.1038/srep25312
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发表时间：
2016-05-04
影响因子：
4.6
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Fang L;Li X;Li L;Li S;Liao X;Sun J;Liu Y
近年来，人们对暴露于重金属，特别是铜和锌的细菌中抗生素耐药性的共同选择表示关切，这些重金属被用作某些牲畜物种的生长促进剂。在本研究中，发现25个含有oqxAB（20/25）/blaCTX-M（18/25）的IncHI 2质粒，其大小在1.260至1.350 kb之间，其中22个属于ST 3-IncHI 2组。除了blaCTX-M和oqxAB外，还在这些IncHI 2质粒上鉴定了pcoA-E（5/25）和silE-P（5/25），以及aac（6′）-Ib-cr（18/25）、floR（16/25）、rmtB（6/25）、qnrS 1（3/25）和fosA 3（2/25）。携带pco和sil的质粒有助于提高CuSO_4和AgNO_3的MIC。除了pco操纵子内的一些变化外，这两个操纵子周围的遗传背景是很保守的。在不同肠杆菌科物种的染色体中也发现了包含IncHI 2质粒中鉴定的两个操纵子的~32 kb区域。系统发育分析表明，Tn 7-like转座子可能在肠杆菌科细菌的sil和pco操纵子的跨属转移中发挥重要作用。结论：pcO和sil操纵子、oqxAB/blaCTX-M以及其他抗生素耐药基因在IncHI 2质粒上的共存可能促进多重耐药菌的产生。
Concerns have been raised in recent years regarding co-selection for antibiotic resistance among bacteria exposed to heavy metals, particularly copper and zinc, used as growth promoters for some livestock species. In this study, 25 IncHI2 plasmids harboring oqxAB (20/25)/blaCTX-M (18/25) were found with sizes ranging from ∼260 to ∼350 kb and 22 belonged to the ST3-IncHI2 group. In addition to blaCTX-M and oqxAB, pcoA-E (5/25) and silE-P (5/25), as well as aac(6′)-Ib-cr (18/25), floR (16/25), rmtB (6/25), qnrS1(3/25) and fosA3 (2/25), were also identified on these IncHI2 plasmids. The plasmids carried pco and sil contributed to increasing in the MICs of CuSO4 and AgNO3. The genetic context surrounding the two operons was well conserved except some variations within the pco operon. The ~32 kb region containing the two operons identified in the IncHI2 plasmids was also found in chromosomes of different Enterobacteriaceae species. Further, phylogenetic analysis of this structure showed that Tn7-like transposon might play an important role in cross-genus transfer of the sil and pco operons among Enterobacteriaceae. In conclusion, co-existence of the pco and sil operons, and oqxAB/blaCTX-M as well as other antibiotic resistance genes on IncHI2 plasmids may promote the development of multidrug-resistant bacteria.