Differentiation-defective stem cells outcompete normal stem cells for niche occupancy in the Drosophila ovary
Differentiation-defective stem cells outcompete normal stem cells for niche occupancy in the Drosophila ovary
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DOI：
10.1016/j.stem.2007.10.021
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发表时间：
2008-01-01
期刊：
影响因子：
23.9
通讯作者：
Xie, Ting
中科院分区：
文献类型：
--
作者：
Jin, Zhigang;Kirilly, Daniel;Xie, Ting
最近在小生境如何控制干细胞功能方面取得了快速进展，但对同一小生境中的干细胞如何相互作用知之甚少。在这项研究中，我们表明，分化缺陷的果蝇卵巢生殖干细胞（GSCs）可以竞争正常的钙粘蛋白依赖的方式占据的生态位。分化缺陷的bam或bgcn突变GSC侵入邻近野生型GSC的生态位空间，并通过上调E-钙粘蛋白表达逐渐将它们推出生态位。此外，bam/bgcn介导的GSC竞争需要E-cadherin和正常的GSC分裂，而不是促进自我更新的BMP小生境信号，而不同的E-cadherin水平可以充分刺激GSC竞争。因此，我们认为GSC在生态位占据方面存在竞争关系，这可以作为一种质量控制机制，确保意外分化的干细胞迅速从生态位中去除并被功能性干细胞取代。
Rapid progress has recently been made regarding how the niche controls stem cell function, but little is yet known about how stem cells in the same niche interact with one another. In this study, we show that differentiation-defective Drosophila ovarian germline stem cells (GSCs) can outcompete normal ones for niche occupancy in a cadherin-dependent manner. The differentiation-defective bam or bgcn mutant GSCs invade the niche space of neighboring wildtype GSCs and gradually push them out of the niche by upregulating E-cadherin expression. Furthermore, the bam/bgcn-mediated GSC competition requires E-cadherin and normal GSC division, but not the self-renewal-promoting BMP niche signal, while different E-cadherin levels can sufficiently stimulate GSC competition. Therefore, we propose that GSCs have a competitive relationship for niche occupancy, which may serve as a quality control mechanism to ensure that accidentally differentiated stem cells are rapidly removed from the niche and replaced by functional ones.