Optical coherence tomography of malignancy in hamster cheek pouches
Optical coherence tomography of malignancy in hamster cheek pouches
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DOI：
10.1117/1.1783897
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发表时间：
2004-09-01
影响因子：
3.5
通讯作者：
Brenner, M
中科院分区：
文献类型：
--
作者：
Matheny, ES;Hanna, NM;Brenner, M
光学相干断层扫描(OCT)/光学多普勒断层扫描（ODT）提供实时的体内高分辨率（10-mum）组织成像和微血管中实时的空间分辨血流。采用OCT/ODT对诱发性发育不良和恶性肿瘤的仓鼠颊袋进行成像，以评估其在气道恶性肿瘤诊断中的应用潜力。在22只金色叙利亚仓鼠中，0.5% 9,11 0-二甲基-1,2-苯并蒽在10周内诱导右侧颊袋癌变；左边的三个是控制器。使用基于1310纳米宽带超发光二极管的OCT/ODT原型设备，在牺牲前和切除后对脸颊袋进行体内成像。将图像与标准组织病理学进行比较。OCT成像提供了仓鼠颊袋深度1至3mm的良好分辨率和平行的组织学图像。在仓鼠颊袋肿瘤模型中证实了高分辨率功能成像的可行性。ODT能准确检测与癌变相关的血管变化。(C) 2004光学仪器工程师学会。
Optical coherence tomography (OCT)/optical Doppler tomography (ODT) provides real-time in vivo high-resolution (10-mum) imaging of tissues and real-time spatially resolved blood flow in microvasculature. Hamster cheek pouches with induced dysplasia and malignancies were imaged with OCT/ODT to assess the potential for application to airway malignancy. In 22 Golden Syrian hamsters, 0.5% 9,11 0-dimethyl-1,2-benzanthracene induces carcinogenesis over 10 weeks in right side cheek pouches; the left side three served as controls. The cheek pouches are imaged in vivo prior to sacrifice, and in vitro after excision, using a prototype 1310-nm broadband superluminescent diode based OCT/ODT device. Images are compared to standard histopathology. OCT imaging offers good resolution of the hamster cheek pouches to depths of 1 to 3 mm and paralleled histologic images. The feasibility of high-resolution functional imaging is demonstrated in this hamster cheek pouch tumor model. ODT accurately detects vascular change associated with carcinogenesis. (C) 2004 Society of Photo-Optical Instrumentation Engineers.