Scaling of nucleic acid assays on microelectrophoresis array devices: high-dynamic range multi-gene readout from less than ten transcripts.
Scaling of nucleic acid assays on microelectrophoresis array devices: high-dynamic range multi-gene readout from less than ten transcripts.
复制标题
微电泳阵列装置上核酸测定的扩展：从少于十个转录本中进行高动态范围多基因读数。
DOI：
10.1002/elps.200800774
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发表时间：
2009
期刊：
影响因子：
2.9
通讯作者：
Ehrlich,DanielJ
中科院分区：
文献类型：
--
作者：
Ueberfeld,Joern;Ehrlich,DanielJ
In this paper we describe progress in using the prodigious data‐collecting ability of multilane microelectrophoresis instruments to bear on problems in scaled nucleic acid assays. We emphasize compound stacking and solid‐support loading as means to concentrate <100 pg samples for direct injection. Reaction Mapping is applied to readout quantitative polymerase chain reaction gene‐expression and as a way to practically overcome difficulty in interpreting amplification curves of multiplexed quantitative polymerase chain reaction at 20–50 gene/well complexity. We demonstrate multiplexed readout of gene expression over an abundancy range of 9 Log 2 units starting with reverse‐transcribed samples as small as five molecules in each sample.
