A novel combined adjuvant strongly enhances mucosal and systemic immunity to low pathogenic avian influenza after oral immunization in ducks
A novel combined adjuvant strongly enhances mucosal and systemic immunity to low pathogenic avian influenza after oral immunization in ducks
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一种新型组合佐剂可强烈增强鸭口服免疫后对低致病性禽流感的粘膜和全身免疫力。
DOI：
10.3382/ps.2012-03000
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发表时间：
2013-06-01
期刊：
影响因子：
4.4
通讯作者：
Yang, Qian
中科院分区：
文献类型：
--
作者：
Kang, Haihong;Wang, Hongli;Yang, Qian
水禽作为禽流感病毒的天然宿主，在禽流感的发生、传播和地方性传播中发挥着重要作用。为了通过一种简单、无创、无针的途径预防禽流感在水禽中的感染，用禽流感灭活病毒(H9N2，IAIV)与CpG DNA和高剂量葡萄糖联合口服免疫鸭，观察鸭的局部和全身免疫反应。此外，还检测了病毒攻击后的免疫保护作用。口服IAIV联合CpG DNA和葡萄糖后，加强免疫后早期小肠组织中IL-2和IL-6的表达水平明显高于IAIV和单一佐剂免疫后的水平。初次免疫后第3、5、7周，局部肠道组织和血清中的IgA和Ig G抗体水平显著升高。此外，在IAIV和两种佐剂免疫后第三到第七周的血清样本中也检测到了增强的血凝抑制效价。在病毒挑战和传播研究中，事先联合使用IAIV和CpG DNA和葡萄糖可以降低感染鸭的泄殖腔拭子和结肠组织中观察到的病毒滴度，并防止病毒在鸭子之间传播。我们的研究表明，CpG DNA和大剂量葡萄糖的结合可以通过增强局部和系统免疫反应，减少病毒脱落来提高灭活H9N2病毒的免疫效果。
As natural reservoirs of avian influenza viruses, waterfowl play an important role in the generation, spread, and enzootic transmission of avian influenza. To prevent avian influenza in waterfowl through a simple, noninvasive, and needle-free route, ducks were immunized orally with an inactivated avian influenza virus (H9N2, IAIV) combined with CpG DNA and high-dose glucose, and then the local and systemic immune responses of these ducks were investigated. In addition, the immune protection was assayed after viral challenge. After the oral administration of IAIV combined with CpG DNA and glucose, the expression levels of interleukin-2 and interleukin-6 in the small intestine tissues increased significantly in the early period after booster immunization relative to the levels after immunization with IAIV and a single adjuvant. Significant increases were also observed in the IgA and IgG antibody levels in the local intestinal tract tissues and serum at wk 3, 5, and 7 after the first immunization. Furthermore, enhanced hemagglutination inhibition titers were also detected in serum samples taken between the third and seventh weeks after immunization with IAIV and both adjuvants. In the viral challenge and transmission study, the prior administration of IAIV combined with both CpG DNA and glucose reduced the viral titers observed for the cloaca swabs and colon tissues of challenged ducks and prevented virus transmission between ducks. Our study suggests that the combination of CpG DNA and high-dose glucose can improve immunization with inactivated H9N2 virus by enhancing the local and systemic immune responses and reducing viral shedding.