Disposable electrochemical immunosensor for carcinoembryonic antigen using ferrocene liposomes and MWCNT screen-printed electrode.
Disposable electrochemical immunosensor for carcinoembryonic antigen using ferrocene liposomes and MWCNT screen-printed electrode.
复制标题
DOI：
10.1016/j.bios.2008.10.006
复制
发表时间：
2009-03
影响因子：
12.6
通讯作者：
S. Viswanathan;C. Rani;Annadurai Vijay Anand;J. Ho
中科院分区：
文献类型：
--
作者：
S. Viswanathan;C. Rani;Annadurai Vijay Anand;J. Ho
研制了检测唾液和血清中癌胚抗原(CEA)的一次性电化学免疫传感器。将抗癌胚抗原单抗(α-CEA)共价固定在聚乙烯亚胺包裹的多壁碳纳米管丝网印刷电极上。以癌胚抗原和α癌胚抗原标记的二茂铁羧酸脂质体(αCEA-FCL)为载体，建立夹心免疫分析方法。方波伏安法(SWV)用于分析免疫偶联脂质体在电极表面释放的二茂铁羧酸的法拉第氧化还原响应。SWV峰电流的大小与CEA的浓度直接相关。癌胚抗原浓度在5×10−12~5×10−7gmL−1范围内线性关系良好，检出限为1×10−12gmL−1(S/N=3)。该方法对人血清和唾液中CEA的测定具有较高的精密度和灵敏度。
Disposable electrochemical immunosensor for the detection of carcinoembryonic antigen (CEA) in saliva and serum was developed. Monoclonal anti-CEA antibodies (αCEA) were covalently immobilized on polyethyleneimine wrapped multiwalled carbon nanotubes screen-printed electrode. A sandwich immunoassay was performed with CEA and αCEA tagged ferrocene carboxylic acid encapsulated liposomes (αCEA-FCL). The square wave voltammetry (SWV) was employed to analyze faradic redox responses of the released ferrocene carboxylic acid from the immunoconjugated liposomes on the electrode surface. The magnitude of the SWV peak current was directly related to the concentration of CEA. The calibration curve for CEA concentration was in the range of 5×10−12to 5×10−7gmL−1with a detection limit of 1×10−12gmL−1(S/N=3). This method provides a high precise and sensitive determination of CEA in human blood serum and saliva samples.