On the molecular basis and regulation of cellular capacitative calcium entry: Roles for Trp proteins
On the molecular basis and regulation of cellular capacitative calcium entry: Roles for Trp proteins
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DOI：
10.1073/pnas.93.26.15195
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发表时间：
1996-12-24
影响因子：
11.1
通讯作者：
Birnbaumer, M
中科院分区：
文献类型：
--
作者：
Birnbaumer, L;Zhu, X;Birnbaumer, M
在过去的两年中，我们的实验室致力于阐明电容性钙进入细胞（CCE）的分子基础。具体来说，我们测试了 CCE 通道是由与果蝇 trp 基因相关的基因编码的亚基形成的假设，这一追求的第一步是寻找哺乳动物 frp 基因，我们发现了不是一个而是六个哺乳动物基因，并克隆了它们的几个 cDNA，其中一些是全长的。正如在哺乳动物细胞中检测的那样，一些哺乳动物 Trp 的过度表达会增加 CCE，而反义方向的部分 trp cDNA 的表达会干扰内源性 CCE。这些发现提供了 CCE 和哺乳动物色氨酸之间的牢固联系。本文回顾了 CCE 的已知形式，并重点介绍了我们对细胞内 Ca2+ 稳态和 CCE 生理作用的理解中尚未解答的问题。
During the last 2 years, our laboratory has worked on the elucidation of the molecular basis of capacitative calcium entry (CCE) into cells. Specifically, we tested the hypothesis that CCE channels are formed of subunits encoded in genes related to the Drosophila trp gene, The first step in this pursuit was to search for mammalian frp genes, We found not one but six mammalian genes and cloned several of their cDNAs, some in their full length, As assayed in mammalian cells, overexpression of some mammalian Trps increases CCE, while expression of partial trp cDNAs in antisense orientation can interfere with endogenous CCE. These findings provided a firm connection between CCE and mammalian Trps. This article reviews the known forms of CCE and highlights unanswered questions in our understanding of intracellular Ca2+ homeostasis and the physiological roles of CCE.