SERIAL SECTION ANALYSIS OF PEROXISOMAL SHAPE AND MEMBRANE RELATIONSHIPS IN THE MOUSE PREPUTIAL GLAND *
SERIAL SECTION ANALYSIS OF PEROXISOMAL SHAPE AND MEMBRANE RELATIONSHIPS IN THE MOUSE PREPUTIAL GLAND *
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小鼠包皮腺过氧化物酶体形状和膜关系的连续切片分析*
DOI：
10.1111/j.1749-6632.1982.tb21468.x
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发表时间：
1982
影响因子：
5.2
通讯作者：
Karin Gorgas
中科院分区：
文献类型：
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作者：
Karin Gorgas
虽然一般认为过氧化物酶体来自内质网的扩张区域（综述见参考文献I），但这两个区室之间的膜连续性从未得到令人信服的说明。大多数已发表的电子显微照片都证明了由过氧化物酶体产生的专门的衰减“尾巴”，这些尾巴与光滑ER的轮廓表现出惊人的形态相似性。然而，这些联系尚未在连续的章节中得到彻底的分析。因此，它是不知道，他们是否真的代表顺利ER通道或薄延长过氧化物酶1节段连接两个相当远的部分相同的细胞器。鉴于关于过氧化物酶体生物发生的明显矛盾的结论，我们研究了在碱性DAB培养基中孵育的小鼠包皮腺的一系列切片（每个系列80-100个切片）。这种修饰的皮脂腺特别参与醚甘油脂的生物合成，“在全分泌分泌周期中表现出过氧化物酶体的大量增殖3以及相当程度的过氧化物酶体多形性”。3
Although it is generally assumed that peroxisomes arise from dilated regions of endoplasmic reticulum (for reviews see ref. I), membrane continuities between the two compartments have never been illustrated convincingly. Most of the published electron micrographs have demonstrated specialized attenuated “tails” arising from peroxisomes which display striking morphological similarities with profiles of smooth ER. However, these connections have not been analyzed thoroughly as yet in serial sections. Consequently, it is not known, whether they indeed represent smooth ER channels or thin extended peroxisoma1 segments connecting two quite distant portions of the same organelle. In view of the apparent contradictory conclusions regarding the biogenesis of peroxisomes we investigated series of ultrathin sections (80-100 sections per each series] of the mouse preputial gland incubated in alkaline DAB medium. This modified sebaceous gland, which is involved particularly in the biosynthesis of ether glycerolipids,’ exhibits a massive proliferation of peroxisomes3 as well as a considerable degree of peroxisomal pleomorphism during the holocrine secretory cycle.‘.3