Microbubble-induced increase in ablation of liver tumors by high-intensity focused ultrasound
Microbubble-induced increase in ablation of liver tumors by high-intensity focused ultrasound
复制标题
DOI：
10.1016/j.hepres.2006.08.013
复制
发表时间：
2006-12-01
影响因子：
4.2
通讯作者：
Matsumoto, Yoichiro
中科院分区：
文献类型：
--
作者：
Hanajiri, Kazuyuki;Maruyama, Toshiyuki;Matsumoto, Yoichiro
我们研究了高强度聚焦超声(HIFU)与微泡剂联合治疗肝细胞癌的可能性。将肝肿瘤大鼠麻醉，行开腹手术，静脉注射微泡剂Levovist或生理盐水。注射结束后，用HIFU照射肝脏30s(2.18 MHz，600W/cm(2)，直径40 mm)。在HIFU照射后即刻，评估HIFU区域的超声图像。HIFU后高回声区的平均体积如下(mm(3)，Levovist对生理盐水：355.3+/-180.7 vs47.4+/-35.6P<；0.001，n=13)。经HIFU治疗的肝组织体积如下(mm(3)，Levovist与生理盐水：275.3+/-120.0 vs60.1+/-23.6P<；0.001，n=13)。HIFU照射区显微镜下可见内爆性囊泡，许多癌细胞被完全破坏(细胞膜或细胞核丢失)。结论：微泡剂Levovist可增加HIFU凝固组织的体积。(C)2006爱思唯尔爱尔兰有限公司。保留所有权利。
We studied the possibility of using high-intensity focused ultrasound (HIFU) together with a microbubble agent to treat hepatocellular carcinoma.Development of liver tumors in rats was induced by administration of Dimethylnitrosamin ( 100 ppm). Rats with liver tumors were anesthetized, underwent laparotomy, and were given the microbubble agent Levovist or saline intravenously. After the injection, the liver was exposed to HIFU for 30s (2.18 MHz, 600 W/cm(2), 40mm in diameter). Immediately after HIFU exposure, ultrasound images of the HIFU area were evaluated. Then the liver was excised and the volume of coagulated tissue was measured.The mean volumes of hyperechoic areas after HIFU were as follows (mm(3), Levovist versus saline: 355.3 +/- 180.7 versus 47.4 +/- 35.6, P < 0.001, n = 13). The volumes of liver tissue coagulated by HIFU were as follows ( mm(3), Levovist versus saline: 275.3 +/- 120.0 versus 60.1 +/- 23.6, P < 0.001, n = 13). On microscopic examination of areas exposed to HIFU, implosion cysts were seen, and many cancer cells were found to have been destroyed completely ( loss of cell membranes or nuclei).In conclusion, the microbubble agent Levovist can increase the volume of tissue coagulated by HIFU. (c) 2006 Elsevier Ireland Ltd. All rights reserved.