The potential for elemental analysis in biotechnology
The potential for elemental analysis in biotechnology
复制标题
DOI：
10.1039/b201494g
复制
发表时间：
2002-01-01
影响因子：
3.4
通讯作者：
Tanner, SD
中科院分区：
文献类型：
--
作者：
Baranov, VI;Quinn, ZA;Tanner, SD
电感耦合等离子体质谱为区别于有机质谱学的方法带来了独特的属性：对伴随的有机或金属物种相对不敏感，对所有类似标记的络合物具有同等的灵敏度，固有的提供绝对定量和响应线性的能力。所有这些积极的特征都符合现代生物技术的要求。本文从蛋白质磷酸化状态的确定、生物活性物质的质量控制和细胞系转染率的控制等几个生物技术迫切需要解决的问题入手，探讨了电感耦合等离子体质谱(ICMS)的应用前景。
The ICP-MS brings particular attributes to the method that distinguishes it from organic mass spectrometry: relative insensitivity to concomitant organic or metallic species, equivalent sensitivity to all similarly-tagged complexes, inherent ability to provide absolute quantitation and linearity of response. All these positive features fit the requirements of modern-day biotechnology. In this work, the potential of ICP-MS is discussed in the context of several urgent problems of biotechnology including: the determination of the state of phosphorylation of proteins, quality control of biologically active materials and control of efficiency of cell line transfections.