Isolation of Retinal Exosome Biomarkers from Blood by Targeted Immunocapture
Isolation of Retinal Exosome Biomarkers from Blood by Targeted Immunocapture
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DOI：
10.1007/978-3-030-27378-1_4
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发表时间：
2019-01-01
期刊：
影响因子：
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通讯作者：
Rickman, Catherine Bowes
中科院分区：
文献类型：
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作者：
Klingeborn, Mikael;Skiba, Nikolai P.;Rickman, Catherine Bowes
视网膜色素上皮（RPE）形成外血-视网膜屏障，提供营养，循环视觉色素，从光感受器中去除废盘，以及许多其他功能。由于这些在视觉稳态中的关键作用，RPE是年龄相关性黄斑变性（AMD）疾病相关变化的主要部位，强调了其在视觉健康和疾病研究中的重要性。不幸的是，没有AMD或疾病进展的早期指标，这一空白可以通过早期AMD生物标志物的发展来填补。外泌体是纳米级大小的脂质双层膜囊泡，由细胞以受控的方式释放，并执行许多细胞外和细胞间活动。在RPE中，它们从顶侧和基侧释放，每个源都有独特的特征/内容。RPE细胞基底外侧释放的外泌体通过脉络膜进入体循环，因此代表了血液中视网膜疾病生物标志物的潜在来源。在这里，我们讨论了眼睛来源的外泌体和其他血液中的小细胞外囊泡的靶向免疫捕获在眼病生物标志物发现中的潜力。
The retinal pigmented epithelium (RPE) forms the outer blood-retinal barrier, provides nutrients, recycles visual pigment, and removes spent discs from the photoreceptors, among many other functions. Because of these critical roles in visual homeostasis, the RPE is a principal location of disease-associated changes in age-related macular degeneration (AMD), emphasizing its importance for study in both visual health and disease. Unfortunately, there are no early indicators of AMD or disease progression, a void that could be filled by the development of early AMD biomarkers. Exosomes are lipid bilayer membrane vesicles of nanoscale sizes that are released in a controlled fashion by cells and carry out a number of extra- and intercellular activities. In the RPE they are released from both the apical and basal sides, and each source has a unique signature/content. Exosomes released from the basolateral side of RPE cells enter the systemic circulation via the choroid and thus represent a potential source of retinal disease biomarkers in blood. Here we discuss the potential of targeted immunocapture of eye-derived exosomes and other small extracellular vesicles from blood for eye disease biomarker discovery.