Effects of the biological nitrification inhibitor 1,9-decanediol on nitrification and ammonia oxidizers in three agricultural soils
Effects of the biological nitrification inhibitor 1,9-decanediol on nitrification and ammonia oxidizers in three agricultural soils
复制标题
生物硝化抑制剂1,9-癸二醇对三种农业土壤硝化作用和氨氧化剂的影响
DOI：
10.1016/j.soilbio.2018.11.008
复制
发表时间：
2019-02-01
影响因子：
9.7
通讯作者：
Shi, Weiming
中科院分区：
文献类型：
--
作者：
Lu, Yufang;Zhang, Xiaonan;Shi, Weiming
生物硝化抑制剂的应用被认为是减轻农业土壤氮素流失的重要新策略。1，9-癸烷醇是最近在水稻根系分泌物中发现的一种新的BNI，并在利用亚硝化单胞菌的生物测定中显示出抑制硝化作用。然而，这种化合物对土壤中硝化作用和氨氧化剂的影响仍然未知。以3种典型农业土壤为材料，通过14 d的微宇宙培养，研究了1，9-癸烷对硝化和氨氧化菌的影响。高剂量的1，9-癸烷醇对三种土壤的硝化作用均有较强的抑制作用，在酸性红壤、碱性潮土和中性水稻土中分别抑制了58.1%、37.0%和35.7%。此外，1，9-癸烷醇的抑制作用上级广泛使用的合成硝化抑制剂双氰胺（DCD）和其他两种BNI，3-（4-羟基苯基）丙酸甲酯（MHPP）和α-亚麻酸（LN），在所有三种土壤。添加1，9-癸烷醇对3种土壤中氨氧化细菌（AOB）和氨氧化古菌（AOA）的丰度均有显著抑制作用。所有AOB序列都属于亚硝化螺菌属，优势AOA序列属于亚硝化球菌属。施用1，9-癸烷酮后，AOB群落组成的变化比AOA更明显。AOB群落结构从亚硝化螺旋菌群2和3a向亚硝化螺旋菌群8a和8b转移。AOA对潮土和水稻土中亚硝化球菌优势类群的比例没有显著影响，而红壤中仅亚硝化藻优势类群的比例有所下降。1，9-癸烷醇也能显著降低土壤N2 O排放，尤其是酸性红壤。我们的研究结果提供的证据表明，1，9-癸烷能够抑制硝化作用在农业土壤中，通过阻碍AOA和AOB，而不是影响土壤NH 4+的有效性。1，9-癸烷醇有望成为土壤氨氧化细菌和古菌的有效生物硝化抑制剂。
The application of biological nitrification inhibitors (BNIs) is considered an important new strategy to mitigate nitrogen losses from agricultural soils. 1,9-decanediol was recently identified as a new BNI in rice root exudates and was shown to inhibit nitrification in bioassays using Nitrosomonas. However, the effect of this compound on nitrification and ammonia oxidizers in soils remained unknown. In this study, three typical agriculture soils were collected to investigate the impact of 1,9-decanediol on nitrification and ammonia oxidizers in a 14-day microcosm incubation. High doses of 1,9-decanediol showed strong soil nitrification inhibition in all three agricultural soils, with the highest inhibition of 58.1% achieved in the acidic red soil, 37.0% in the alkaline fluvoaquic soil, and 35.7% in the neutral paddy soil following 14 days of incubation. Moreover, the inhibition of 1,9-decanediol was superior to the widely used synthetic nitrification inhibitor, dicyandiamide (DCD) and two other BNIs, methyl 3-(4-hydroxyphenyl) propionate (MHPP) and alpha-linolenic acid (LN), in all three soils. The abundance of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) was significantly inhibited by 1,9-decanediol addition across the three soils. All AOB sequences fell within the Nitrosospira group, and the dominant AOA sequences belonged to the Nitrososphaera cluster in all three soils. Changes in the community composition of AOB were more pronounced than AOA after the application of 1,9-decanediol. The AOB community structure shifted from Nitrosospira cluster 2 and cluster 3a toward Nitrosospira clusters 8a and 8b. As for AOA, no significant impact on the proportion of the dominant Nitrososphaera cluster was observed in the fluvoaquic soil and paddy soil while only the Nitrosopwralus cluster decreased in the red soil. 1,9-decanediol could also significantly reduce soil N2O emissions, especially in acidic red soil. Our results provide evidence that 1,9-decanediol is capable of suppressing nitrification in agricultural soils through impeding both AOA and AOB rather than affecting soil NH4+ availability. 1,9-decanediol holds promise as an effective biological nitrification inhibitor for soil ammonia-oxidizing bacteria and archaea.