A structure of the COPI coat and the role of coat proteins in membrane vesicle assembly
A structure of the COPI coat and the role of coat proteins in membrane vesicle assembly
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DOI：
10.1126/science.aab1121
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发表时间：
2015-07-10
期刊：
影响因子：
56.9
通讯作者：
Briggs, J. A. G.
中科院分区：
文献类型：
--
作者：
Dodonova, S. O.;Diestelkoetter-Bachert, P.;Briggs, J. A. G.
细胞内物质的运输是由从一个细胞区室萌发并与另一个细胞区室融合的运输囊泡介导的。运输囊泡的形成是由膜层驱动的，膜层将货物定位并聚合成笼子以弯曲膜。尽管可以获得这些涂层成分的广泛结构信息，但运输囊泡的异质性阻碍了对完整膜涂层如何在膜上组装的理解。我们结合了冷冻电子断层扫描、亚断层扫描平均和交联质谱分析，得出了参与高尔基体和内质网之间交通的组装外壳蛋白复合物 I (COPI) 外壳的完整模型。高度互连的 COPI 涂层结构与当前对涂层囊泡形成的“适配器和笼”理解相矛盾。
Transport of material within cells is mediated by trafficking vesicles that bud from one cellular compartment and fuse with another. Formation of a trafficking vesicle is driven by membrane coats that localize cargo and polymerize into cages to bend the membrane. Although extensive structural information is available for components of these coats, the heterogeneity of trafficking vesicles has prevented an understanding of how complete membrane coats assemble on the membrane. We combined cryo-electron tomography, subtomogram averaging, and cross-linking mass spectrometry to derive a complete model of the assembled coat protein complex I (COPI) coat involved in traffic between the Golgi and the endoplasmic reticulum. The highly interconnected COPI coat structure contradicted the current "adaptor-and-cage" understanding of coated vesicle formation.