ANALYSIS OF THE SPECIFICITY OF 5 MURINE ANTI-BLOOD GROUP-A MONOCLONAL-ANTIBODIES, INCLUDING ONE THAT IDENTIFIES TYPE-3 AND TYPE-4 A-DETERMINANTS
ANALYSIS OF THE SPECIFICITY OF 5 MURINE ANTI-BLOOD GROUP-A MONOCLONAL-ANTIBODIES, INCLUDING ONE THAT IDENTIFIES TYPE-3 AND TYPE-4 A-DETERMINANTS
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DOI：
10.1021/bi00347a047
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发表时间：
1985-12-17
期刊：
影响因子：
2.9
通讯作者：
LLOYD, KO
中科院分区：
文献类型：
--
作者：
FURUKAWA, K;CLAUSEN, H;LLOYD, KO
5种小鼠抗A血型单抗，其中4种是用培养的人癌细胞免疫产生的，1种是用人工合成的抗原产生的，通过检测它们与纯化的A糖脂、红细胞糖脂、低聚糖、卵巢囊糖蛋白和唾液糖蛋白的反应性，确定了它们的特异性。其中两个抗体(HT29-36和CB)与所测试的所有A变异体结构发生反应，并具有广泛的抗A反应活性。AB CLH6不凝集A型红细胞，优先与1A型结构反应。AB S12能凝集所有A1红细胞，与AA-2、Ab-2和A四糖等简单的单糖2A型结构反应最好。AB M2有一个新颖但复杂的反应性光谱。它与3型和4A型链反应，而不与1型和2A型链反应。它似乎既识别外部的A结构。**图形**。(I)(存在于类型3和类型4链中)以及还存在于类型3链中的内部结构(Ii)。AB M2可凝集所有的A和AB红细胞，但不与唾液糖蛋白反应。
The specificity of five mouse monoclonal anti-A blood group antibodies (Ab), four of which were produced by immunization with cultured human cancer cells and one with a synthetic antigen, has been determined by examining their reactivity with purified A glycolipids, erythrocyte glycolipids, oligosaccharides, ovarian cyst glycoproteins, and salivary glycoproteins. Two of the antibodies (HT29-36 and CB) reacted with all A variant structures tested and have a broad anti-A reactivity. Ab CLH6 did not agglutinate A erythrocytes and reacted preferentially with the type 1A structure. Ab S12 agglutinated all A1 erythrocytes and reacted best with simple, monofucosyl type 2 A structures, such as Aa-2, Ab-2, and A tetrasaccharide. Ab M2 has a novel, but complex, spectrum of reactivity. It reacts with type 3 and type 4 A chains and not with type 1 and type 2 A chains. It appears to recognize both an external A structure .**GRAPHIC**. (I) (found in type 3 and type 4 chains) and also an internal structure (II) found in type 3 chains. Ab M2 agglutinates all A and AB erythrocytes but does not react with salivary glycoproteins.