Rock geochemistry induces stress and starvation responses in the bacterial proteome
Rock geochemistry induces stress and starvation responses in the bacterial proteome
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DOI：
10.1111/1462-2920.13093
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发表时间：
2016-04-01
影响因子：
5.1
通讯作者：
Cockell, Charles S.
中科院分区：
文献类型：
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作者：
Bryce, Casey C.;Le Bihan, Thierry;Cockell, Charles S.
微生物与岩石之间的相互作用在地球系统过程中起着重要作用。然而，人们对微生物在岩石环境中生存所需的分子能力知之甚少。利用定量无标记蛋白质组学方法，研究人员发现一种模式细菌（Cupriavidus metalliduransCH34）可以利用火山岩来满足一些元素需求，从而在镁和铁限制的培养基中增加细胞分裂率。然而，岩石也通过与pH、元素浸出和营养物质表面吸附相关的化学变化引入了多种新的压力，这些变化反映在蛋白质组中。例如，观察到生物可利用磷的损失，并导致多种磷酸盐限制蛋白的上调，这有助于增加细胞内的磷酸盐摄取和清除。我们的研究结果表明，尽管提供了必需的元素，岩石化学驱动岩石生物体内复杂的代谢重组，需要在蛋白质水平上严格调节细胞过程。这项研究提高了我们识别关键微生物反应的能力，这些反应使生命能够在岩石环境中持续存在。
Interactions between microorganisms and rocks play an important role in Earth system processes. However, little is known about the molecular capabilities microorganisms require to live in rocky environments. Using a quantitative label-free proteomics approach, we show that a model bacterium (Cupriavidus metalliduransCH34) can use volcanic rock to satisfy some elemental requirements, resulting in increased rates of cell division in both magnesium- and iron-limited media. However, the rocks also introduced multiple new stresses via chemical changes associated with pH, elemental leaching and surface adsorption of nutrients that were reflected in the proteome. For example, the loss of bioavailable phosphorus was observed and resulted in the upregulation of diverse phosphate limitation proteins, which facilitate increase phosphate uptake and scavenging within the cell. Our results revealed that despite the provision of essential elements, rock chemistry drives complex metabolic reorganization within rock-dwelling organisms, requiring tight regulation of cellular processes at the protein level. This study advances our ability to identify key microbial responses that enable life to persist in rock environments.