A novel male-sterile mutant of Arabidopsis thaliana, faceless pollen-1, produces pollen with a smooth surface and an acetolysis-sensitive exine
A novel male-sterile mutant of Arabidopsis thaliana, faceless pollen-1, produces pollen with a smooth surface and an acetolysis-sensitive exine
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DOI：
10.1023/b:plan.0000009269.97773.70
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发表时间：
2003-09-01
影响因子：
5.1
通讯作者：
Toriyama, K
中科院分区：
文献类型：
--
作者：
Ariizumi, T;Hatakeyama, K;Toriyama, K
在拟南芥T-DNA标记品系中发现了一个表现条件性雄性不育的突变体，其育性在高湿度条件下得以恢复。扫描电子显微镜(SEM)显示，花粉表面基本光滑，网纹不明显。因此，该突变体被命名为无脸花粉-1(Flp1)。透射电子显微镜分析表明，这种光滑的外观是由于酪氨酸填充在外壁腔中并覆盖在花粉表面。突变体花粉中的脂滴比野生型的更小、更多。扫描电子显微镜分析还表明，花粉外壁很容易被乙酰化破坏，这表明突变体中的外壁成分孢粉蛋白存在缺陷。此外，茎和角果的蜡晶数量减少。预测的与标记基因flp1对应的cDNA氨基酸序列与参与蜡质生物合成的蛋白质序列相似。Flp1蛋白可能在酪氨酸、外壁孢粉蛋白以及茎和角果蜡质的合成中起作用。
A mutant exhibiting conditional male sterility, in which fertility was restored under conditions of high humidity, was identified in T-DNA tagged lines of Arabidopsis thaliana. Scanning electron microscopy (SEM) demonstrated that the pollen surface was almost smooth and the reticulate pattern not prominent. Thus, the mutant was named faceless pollen-1 (flp1). Transmission electron microscopy (TEM) revealed that the smooth appearance was due to tryphine filling in the exine cavities and covering the pollen surface. The lipid droplets in the tryphine of mutant pollen were smaller and more numerous than those of the wild type. SEM analysis also demonstrated that pollen exine was easily damaged by acetolysis, suggesting that a component of exine, sporopollenin, was defective in the mutant. In addition, the stems and siliques had reduced amounts of wax crystals. A predicted amino acid sequence of the cDNA that corresponded to the tagged gene, flp1, showed sequence similarity to proteins involved in wax biosynthesis. The FLP1 protein is likely to play a role in the synthesis of the components of tryphine, sporopollenin of exine and the wax of stems and siliques.