Engineering vascular networks in porous polymer matrices
Engineering vascular networks in porous polymer matrices
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DOI：
10.1002/jbm.10134
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发表时间：
2002-06-15
期刊：
影响因子：
--
通讯作者：
Mooney, DJ
中科院分区：
文献类型：
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作者：
Peters, MC;Polverini, PJ;Mooney, DJ
增强的血管化对于缺血组织的治疗以及新组织和器官的工程化至关重要。我们研究了血管内皮生长因子（VEGF）的持续和局部输送与人微血管内皮细胞（HMVEC）移植相结合是否可以用于设计新的血管网络。 VEGF 以持续的方式从多孔聚乳酸-乙醇酸 (PLG) 基质中掺入并释放，以促进移植部位的血管生成。 VEGF 可以以生物活性形式从 PLG 基质中掺入和释放，其中大部分 VEGF 释放 (64%) 在 2 周内发生。这些基质促进植入 7 天后侵入基质的宿主 SCID 小鼠来源毛细血管的密度增加 260%，证实了释放的 VEGF 的活性。将 HMVEC 移植到 PLG 基质上的 SCID 小鼠中，并在 3 天内组织形成未成熟的人源血管。 7天内观察功能血管。重要的是，当 HMVEC 移植到 VEGF 释放基质上时，14 天后观察到人源血管密度增加了 160%。这些发现表明，将血管生成和血管生成的要素相结合提供了一种增强局部血管化的可行且新颖的方法。 (C) 2002 年 Wiley 期刊公司
Enhanced vascularization is critical to the treatment of ischemic tissues and the engineering of new tissues and organs. We have investigated whether sustained and localized delivery of vascular endothelial growth factor (VEGF) combined with transplantation of human microvascular endothelial cells (HMVECs) can be used to engineer new vascular networks. VEGF was incorporated and released in a sustained manner from porous poly(lactic-co-glycolic acid) (PLG) matrices to promote angiogenesis at the transplantation site. VEGF could be incorporated and released in a biologically active form from PLG matrices with the majority of VEGF release (64%) occurring within 2 weeks. These matrices promoted a 260% increase in the density of host SCID mouse-derived capillaries invading the matrices after 7 days of implantation, confirming the activity of the released VEGF. HMVECs were transplanted into SCID mice on PLG matrices, and organized to form immature human-derived vessels within 3 days. Functional vessels were observed within 7 days. Importantly, when HMVECs were transplanted on VEGF-releasing matrices, a 160% increase in the density of human-derived blood vessels was observed after 14 days. These findings suggest that combining elements of vasculogenesis and angiogenesis provides a viable and novel approach to enhancing local vascularization. (C) 2002 Wiley Periodicals, Inc.