Microbial Biogeography and Core Microbiota of the Rat Digestive Tract.
Microbial Biogeography and Core Microbiota of the Rat Digestive Tract.
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大鼠消化道的微生物生物地理学和核心微生物群
DOI：
10.1038/srep45840
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发表时间：
2017-04-04
影响因子：
4.6
通讯作者：
Chen W
中科院分区：
文献类型：
--
作者：
Li D;Chen H;Mao B;Yang Q;Zhao J;Gu Z;Zhang H;Chen YQ;Chen W
作为一种历史悠久的生物医学模型，大鼠经常被用于探索胃肠道（GI）细菌群与疾病之间的相关性的研究。在本研究中，对沿大鼠消化道纵轴采集的管腔和粘膜样本进行基于 16S rRNA 基因测序的分析，以确定基线微生物组成。结果表明，群落多样性从上消化道段到下消化道段逐渐增加，微生物群落的分层以及微生物代谢物的转移是由生物地理位置驱动的。在胃和小肠中发现了更大比例的产乳酸菌（如乳杆菌、Turicibacter 和链球菌），而厌氧 Lachnospiraceae 和 Ruminococcaceae 发酵碳水化合物和植物芳香化合物，构成了大肠核心微生物群的主体，这些微生物群在拓扑上不同的共存 为相邻的腔室和粘膜室构建了网络。当比较不同宿主的胃肠道微生物群时，我们发现大鼠微生物生物地理学可能代表了一个不同于其他鼠类动物的新参考。我们的研究首次为研究界提供了大鼠胃肠道微生物群景观的全面表征，为更好地理解和预测微生物群落中与疾病相关的变化奠定了基础。
As a long-standing biomedical model, rats have been frequently used in studies exploring the correlations between gastrointestinal (GI) bacterial biota and diseases. In the present study, luminal and mucosal samples taken along the longitudinal axis of the rat digestive tract were subjected to 16S rRNA gene sequencing-based analysis to determine the baseline microbial composition. Results showed that the community diversity increased from the upper to lower GI segments and that the stratification of microbial communities as well as shift of microbial metabolites were driven by biogeographic location. A greater proportion of lactate-producing bacteria (such as Lactobacillus, Turicibacter and Streptococcus) were found in the stomach and small intestine, while anaerobic Lachnospiraceae and Ruminococcaceae, fermenting carbohydrates and plant aromatic compounds, constituted the bulk of the large-intestinal core microbiota where topologically distinct co-occurrence networks were constructed for the adjacent luminal and mucosal compartments. When comparing the GI microbiota from different hosts, we found that the rat microbial biogeography might represent a new reference, distinct from other murine animals. Our study provides the first comprehensive characterization of the rat GI microbiota landscape for the research community, laying the foundation for better understanding and predicting the disease-related alterations in microbial communities.