The LRIG gene family has three vertebrate paralogs widely expressed in human and mouse tissues and a homolog in Ascidiacea
The LRIG gene family has three vertebrate paralogs widely expressed in human and mouse tissues and a homolog in Ascidiacea
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DOI：
10.1016/j.ygeno.2004.01.013
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发表时间：
2004-07-01
期刊：
影响因子：
4.4
通讯作者：
Hedman, H
中科院分区：
文献类型：
--
作者：
Guo, DS;Holmlund, C;Hedman, H
人LRIG1(以前称为LIG1)、人LRIG2和小鼠Lrig1(也称为Lig-1)编码完整的膜蛋白。人类基因位于染色体3p14和1p13，这是人类癌症中经常缺失的区域。我们已经寻找了LRIG家族的其他成员，并通过分子克隆鉴定了人LRIG3及其小鼠直系同源基因Lrig3。人类LRIG3位于染色体12q13。在公共数据库的电子分析中发现了一个小鼠Lrig2 mRNA，在河豚红纹河豚中有三个LRIG同源物，在海鞘中有一个LRIG同源物。人和小鼠的LRIG多肽具有相同的预测结构域结构：一个信号肽，15个富含亮氨酸的串联重复序列，富含半胱氨酸的N-和C-侧翼结构域，三个免疫球蛋白样结构域，一个跨膜区和一个细胞质尾部。胞外部分是最保守的区域，特别是IgC2.2结构域、跨膜区和细胞质尾部的膜近端部分。Northern印迹分析和实时定量RT-PCR分析表明，这三个LRIG同源基因在人和小鼠组织中广泛表达。总之，LRIG基因家族被发现有三个广泛表达的哺乳动物同源基因，在鱼类中有相应的同源基因，在海鞘类中有一个同源基因。(C)2004 Elsevier Inc.保留所有权利。
Human LRIG1 (formerly LIG1), human LRIG2, and mouse Lrig1 (also known as Lig-1) encode integral membrane proteins. The human genes are located at chromosomes 3p14 and 1p13, which are regions frequently deleted in human cancers. We have searched for additional members of the LRIG family and by molecular cloning identified human LRIG3 and its mouse ortholog Lrig3. Human LRIG3 is located at chromosome 12q13. In silico analysis of public databases revealed a mouse Lrig2 mRNA, three LRIG homologs in the puffer fish Fugu rubripes, and one LRIG homolog in the ascidian tunicate Ciona intestinalis. The human and mouse LRIG polypeptides have the same predicted domain organization: a signal peptide, 15 tandem leucine-rich repeats with cysteine-rich N- and C-flanking domains, three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tail. The extracellular part-especially the IgC2.2 domain, the transmembrane domain, and the membrane-proximal part of the cytoplasmic tail-are the most conserved regions. Northern blot analysis and real-time RT-PCR revealed that the three LRIG paralogs are widely expressed in human and mouse tissues. In conclusion, the LRIG gene family was found to have three widely expressed mammalian paralogs, corresponding orthologs in fish, and a homolog in Ascidiacea. (C) 2004 Elsevier Inc. All rights reserved.