Alterations in the Composition of Intestinal DNA Virome in Patients With COVID-19.
Alterations in the Composition of Intestinal DNA Virome in Patients With COVID-19.
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COVID-19 患者肠道 DNA 病毒组组成的变化
DOI：
10.3389/fcimb.2021.790422
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发表时间：
2021
影响因子：
5.7
通讯作者：
Shao ZJ
中科院分区：
文献类型：
--
作者：
Lu ZH;Zhou HW;Wu WK;Fu T;Yan M;He Z;Sun SW;Ji ZH;Shao ZJ
2019冠状病毒病（COVID - 19）患者因感染严重急性呼吸综合征冠状病毒2（SARS - CoV - 2），主要表现出呼吸问题及相关症状，此外消化系统尤其是肠道也受到显著影响。虽然一些研究表明COVID - 19患者的肠道菌群发生了变化，但关于此类患者肠道病毒组的信息却不多。在这项研究中，我们使用基于最新肠道病毒组数据库的ViromeScan软件分析了15例COVID - 19患者的肠道DNA病毒组构成，并研究了其特征性变化，尤其是肠道DNA病毒组的变化，以进一步探究COVID - 19对人体肠道的影响。COVID - 19患者肠道中的DNA病毒主要是类crAss噬菌体（35.48%）、肌尾噬菌体科（20.91%）和长尾噬菌体科（20.43%）病毒。与健康对照组相比，COVID - 19患者的肠道病毒组构成从首次入院时就发生了显著变化，尤其是类crAss噬菌体家族。病毒组和细菌组（如复层噬菌体科和拟杆菌科）的变化之间也显示出潜在的相关性。还通过对因COVID - 19住院的患者肠道进行连续样本采集分析了病毒和细菌种群的丰度。肠道病毒组确实受到SARS - CoV - 2感染的影响，并且与肠道细菌组一起，可能在COVID - 19的疾病进展中发挥重要作用。这些结论将有助于理解肠道相关反应，并有助于COVID - 19的治疗和预防策略。
Patients with Coronavirus Disease 2019 (COVID-19), due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection mainly present with respiratory issues and related symptoms, in addition to significantly affected digestive system, especially the intestinal tract. While several studies have shown changes in the intestinal flora of patients with COVID-19, not much information is available on the gut virome of such patients. In this study, we used the viromescan software on the latest gut virome database to analyze the intestinal DNA virome composition of 15 patients with COVID-19 and investigated the characteristic alternations, particularly of the intestinal DNA virome to further explore the influence of COVID-19 on the human gut. The DNA viruses in the gut of patients with COVID-19 were mainly crAss-like phages (35.48%), Myoviridae (20.91%), and Siphoviridae (20.43%) family of viruses. Compared with healthy controls, the gut virome composition of patients with COVID-19 changed significantly, especially the crAss-like phages family, from the first time of hospital admission. A potential correlation is also indicated between the change in virome and bacteriome (like Tectiviridae and Bacteroidaceae). The abundance of the viral and bacterial population was also analyzed through continuous sample collection from the gut of patients hospitalized due to COVID-19. The gut virome is indeed affected by the SARS-CoV-2 infection, and along with gut bacteriome, it may play an important role in the disease progression of COVID-19. These conclusions would be helpful in understanding the gut-related response and contribute to the treatment and prevention strategies of COVID-19.