Interleukin 8 in human hepatocellular carcinoma correlates with cancer cell invasion of vessels but not with tumor angiogenesis
Interleukin 8 in human hepatocellular carcinoma correlates with cancer cell invasion of vessels but not with tumor angiogenesis
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DOI：
10.1245/aso.2005.07.015
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发表时间：
2005-10-01
影响因子：
3.7
通讯作者：
Aikou, T
中科院分区：
文献类型：
--
作者：
Kubo, F;Ueno, S;Aikou, T
背景：肝细胞癌（HCC）是一种富血供的恶性肿瘤，血管生成因子在HCC的发生发展中起重要作用。本研究探讨了白细胞介素（IL）-8，一个有效的血管生成因子，在HCC samples.Methods的表达：我们测量IL-8的表达，采用逆转录-聚合酶链反应在45例接受手术切除的临床HCC组织。然后我们评估了IL-8表达与微血管生长或临床病理因素之间的相关性。我们还阐明了IL-8对HepG 2的体外增殖，趋化性和invasion.Results的荧光测定法的发展的影响：IL-8的表达并没有显着相关性的HCC组织中的微血管计数，但显微血管浸润的发病率是在IL-8阳性比IL-8阴性组织显着较高。因此，更多的IL-8表达在肝癌病理阶段III/IV比在阶段I/II。体外实验表明，IL-8刺激HepG 2的趋化和侵袭活动，而不是细胞proliferation.Conclusions：IL-8在人肝癌的表达有更多的相关性转移的潜力，如血管浸润，比血管生成或细胞增殖。
Background: Angiogenic factor seems necessary for the development of hepatocellular carcinoma (HCC), which is a hypervascular malignancy. This study examined the expression of interleukin (IL)-8, a potent angiogenic factor, in HCC samples.Methods: We measured IL-8 expression by using reverse transcriptase-polymerase chain reaction in clinical HCC tissues from 45 patients who underwent surgical resection. We then assessed correlations between IL-8 expression and microvessel growth or clinicopathologic factors. We also elucidated the in vitro effect of IL-8 on HepG2 development by using fluorometric assays of proliferation, chemotaxis, and invasion.Results: The expression of IL-8 did not significantly correlate with the microvessel count in HCC tissues, but the incidence of microscopic vessel invasion was significantly higher in IL-8-positive than in IL-8-negative tissues. Thus, more IL-8 was expressed in HCCs at pathologic stage III/IV than in those at stage I/II. Assays in vitro showed that IL-8 stimulates HepG2 chemotactic and invasive activities rather than cell proliferation.Conclusions: The expression of IL-8 in human HCC has more relevance to metastatic potential, such as vessel invasion, than to angiogenesis or cell proliferation.