Platelet deposition on ePTFE grafts coated with fibrin glue with or without FGF-1 and heparin.
Platelet deposition on ePTFE grafts coated with fibrin glue with or without FGF-1 and heparin.
复制标题
血小板沉积在涂有纤维蛋白胶（含或不含 FGF-1 和肝素）的 ePTFE 移植物上。
DOI：
10.1006/jsre.1996.4903
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Greisler,HP
中科院分区：
文献类型：
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作者：
Zarge,JI;Gosselin,C;Huang,P;Vorp,DA;Severyn,DA;Greisler,HP
小口径假体移植物的长期通畅性令人失望，部分原因可能是假体表面的早期血栓形成。我们以前报道过，在动物模型中，用含有成纤维细胞生长因子1（FGF-1）和肝素的纤维蛋白胶（FG）涂覆膨体聚四氟乙烯（ePTFE）加速了ePTFE移植物的自发内皮覆盖;然而，FG对血小板的影响尚不清楚。这项研究是为了评估血小板沉积到FG/FGF-1/肝素涂层与FG涂层与全血preclotted ePTFE surfaces.MethodsTwelve 5厘米ePTFE移植物进行了治疗，无论是与FG（凝血酶，0.32 U/ml，和纤维蛋白原，32.1 mg/ml，n= 8）或FG含有FGF-1（11 ng/ml）加肝素（250 U/ml，n= 4）。用犬（n= 8）或人（n= 4）全血预凝12个对照ePTFE移植物。将这些经处理的移植物成对放置在环路脉动灌注系统上（用FG或FG/FGF-1/肝素预凝），并用M-199/10%FBS/111铟标记的血小板悬浮液灌注。60分钟后，对移植物进行γ计数并测定CPM/mm 2。与用FG/FGF-1/肝素或单独FG处理的移植物相比，预凝的ePTFE移植物显示出类似的血小板沉积增加结论血小板在FG/FGF-1/肝素复合物上的沉积减少，涂层移植物与预凝块移植物的差异不是由于肝素，也不是犬或人血小板所特有的。与全血预凝相比，FG涂层移植物可诱导早期移植物血栓形成性降低。
IntroductionThe disappointing long-term patency of small-caliber prosthetic grafts may be due in part to early thrombogenicity of the prosthetic surface. We previously reported that the coating of expanded polytetrafluoroethylene (ePTFE) with fibrin glue (FG) containing fibroblast growth factor type 1 (FGF-1) and heparin accelerated spontaneous endothelial coverage of ePTFE grafts in an animal model; however, FG's effect on platelets remains unclear. This study was done to evaluate platelet deposition onto FG/FGF-1/heparin-coated vs FG-coated vs whole-blood-preclotted ePTFE surfaces.MethodsTwelve 5-cm ePTFE grafts were treated either with FG (thrombin, 0.32 U/ml, and fibrinogen, 32.1 mg/ml,n= 8) or with FG containing FGF-1 (11 ng/ml) plus heparin (250U/ml,n= 4). Twelve control ePTFE grafts were preclotted with canine (n= 8) or human (n= 4) whole blood. These treated grafts were placed onto a loop pulsatile perfusion system in pairs (preclotted with either FG or FG/FGF-1/heparin) and perfused with a M-199/10% FBS/111indium-labeled platelet suspension. After 60 min the grafts were gamma counted and CPM/mm2were determined.ResultsIn both trials, the preclotted ePTFE grafts demonstrated similarly increased platelet deposition when compared to grafts treated with FG/FGF-1/heparin or FG alone (P< 0.001 for each).ConclusionThe decrease in platelet deposition on the FG/FGF-1/heparin-coated grafts vs preclotted grafts is not due to heparin and is not specific to canine or human platelets. FG-coated grafts may induce a decrease in early graft thrombogenicity when compared to whole blood preclotting.