Determination of UCP1 expression in subcutaneous and perirenal adipose tissues of patients with hypertension
Determination of UCP1 expression in subcutaneous and perirenal adipose tissues of patients with hypertension
复制标题
高血压患者皮下及肾周脂肪组织中UCP1表达的测定
DOI：
10.1007/s12020-015-0572-3
复制
发表时间：
2015-11-01
期刊：
影响因子：
3.7
通讯作者：
Ding, Guoxian
中科院分区：
文献类型：
--
作者：
Li, Xueqin;Liu, Juan;Ding, Guoxian
本研究的目的是确定人肾周脂肪组织（PAT）的性质，并评估高血压患者PAT的脂肪性质。94例患者，包括64例正常血压患者（T-NP）和30例高血压患者（HP），接受肾脏手术。使用定量实时聚合酶链反应、蛋白质印迹和免疫组织化学在PAT和背部皮下脂肪组织（bSAT）库中进行表达分析。与bSAT相比，PAT脂肪细胞体积较小，解偶联蛋白1（UCP 1）mRNA和蛋白质表达明显升高，而经典米色和白色脂肪细胞标志物mRNA表达降低。免疫组化分析显示PAT中多房UCP 1阳性脂肪细胞多于bSAT。UCP 1在HP患者PAT中的表达低于T-NP或年龄和体重指数匹配的NP组。HP组的PAT中UCP 1染色较NP组明显减少，而bSAT中UCP 1染色无明显差异。PAT是一种棕色脂肪。UCP 1在HP患者PAT中的表达低于正常血压患者。UCP 1在PAT中的表达可作为高血压的保护性指标。
The objective of this study is to determine the property of human perirenal adipose tissue (PAT) and assess the adipose property of PAT in hypertension. Ninety-four patients, including 64 normotensive patients (T-NP) and 30 hypertensive patients (HP), who underwent renal surgery were included. Expression analysis was performed using quantitative real-time polymerase chain reaction, Western blot, and immunohistochemistry in PAT and back subcutaneous adipose tissue (bSAT) depots. Compared with bSAT, PAT adipocytes were smaller, and the expressions of uncoupling protein-1 (UCP1) mRNA and protein were markedly higher, while the mRNA expressions of markers for classic beige and white adipocytes were lower in PAT. Immunohistochemistry analysis showed more multilocular UCP1-positive adipocytes in PAT than in bSAT. UCP1 expressions were lower in PAT in HP than in the T-NP or age- and body mass index-matched NP groups. Bigger unilocular adipocytes with less UCP1 staining in PAT were detected in HP than in NP group, although no such difference was observed in bSAT. PAT acts as a brown-like fat. UCP1 expression of PAT was lower in HP than in normotensive patients. UCP1 expression of PAT may serve as a protective indicator for hypertension.