A soft and flexible biosensor using a phospholipid polymer for continuous glucose monitoring
A soft and flexible biosensor using a phospholipid polymer for continuous glucose monitoring
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DOI：
10.1007/s10544-009-9300-1
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发表时间：
2009-08-01
影响因子：
2.8
通讯作者：
Mitsubayashi, Kohji
中科院分区：
文献类型：
--
作者：
Chu, MingXing;Kudo, Hiroyuki;Mitsubayashi, Kohji
利用磷脂聚合物制备了固定化葡萄糖氧化酶（GOD）的柔性生物传感器，并进行了实验研究。首先，用磷脂聚合物（2-甲基丙烯酰氧乙基磷酸胆碱（MPC）与2-乙基己基甲基丙烯酸酯（EHMA）： PMEH）将GOD固定在多孔聚四氟乙烯（PTFE）膜上形成酶膜。根据安培测量结果，优选出GOD的平均固定密度为38.9单位cm（-2）。还研究了温度和pH的依赖性。然后，利用PMEH将GOD固定在柔性过氧化氢电极上，制备了柔性葡萄糖传感器。柔性葡萄糖传感器输出电流与葡萄糖浓度在0.05 ~ 1.00 mmol L-1范围内呈线性关系，相关系数为0.999。标定范围为正常泪液葡萄糖水平0.14-0.23 mmol L-1。这表明，柔性生物传感器被认为是有用的监测泪液中的葡萄糖。
A flexible biosensor using a phospholipid polymer to immobilization of glucose oxidase (GOD) was fabricated and tested. At first, an enzyme membrane formed by immobilizing GOD onto a porous polytetrafluoroethylene (PTFE) membrane using the phospholipid polymer (2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with 2-ethylhexylmethacrylate (EHMA) : PMEH) was evaluated. According to the result of amperometric measurement, average density of GOD to be immobilized was optimized to 38.9 units cm(-2). Temperature and pH dependences were also investigated. Then, a flexible glucose sensor was fabricated by immobilizing GOD onto a flexible hydrogen peroxide electrode using PMEH. The flexible glucose sensor showed a linear relationship between output currents and glucose concentration in 0.05-1.00 mmol L-1, with a correlation coefficient of 0.999. The calibration range covered the normal tear glucose level of 0.14-0.23 mmol L-1. This indicates that the flexible biosensor is considered to be useful for monitoring of glucose in tear fluids.