ADSORPTION OF PROTEIN ON NONSTOICHIOMETRIC CALCIUM STRONTIUM HYDROXYAPATITE
ADSORPTION OF PROTEIN ON NONSTOICHIOMETRIC CALCIUM STRONTIUM HYDROXYAPATITE
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DOI：
10.1016/0927-7757(94)02969-5
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发表时间：
1995-01-20
影响因子：
5.2
通讯作者：
ISHIKAWA, T
中科院分区：
文献类型：
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作者：
KANDORI, K;SAITO, M;ISHIKAWA, T
研究了牛血清白蛋白（BSA）在合成的具有不同钙锶成分和结构的非化学计量羟基磷灰石（CaSrHAP）上的吸附性能。BSA在CaSrHAP颗粒上的吸附等温线为伪langmuir型。饱和吸附量（n(s)）（吸附等温线的平台值）随Sr/(Ca+Sr)比线性增加。同时，n(s)值随着(Ca+Sr)/P摩尔比的增大而增大，说明BSA和CaSrHAP粒子之间的静电吸引力是n(s)的决定因素。然而，尽管阳离子/磷比很大，但两种表面凹凸不平的团聚体粒子的n(s)值较小。这一事实可以用这两种粒子和其他粒子在粒子结构上的差异来解释；表面平坦的大针状颗粒有利于吸附带负电荷的牛白蛋白，因为暴露的ac或be晶体平面上有大量带正电的吸附位点。因此，颗粒的结构对牛血清白蛋白的吸附有重要影响。
The adsorption of bovine serum albumin (BSA) on synthesized non-stoichiometric calcium-strontium hydroxyapatite (CaSrHAP) with various Ca-Sr components and textures was investigated. The adsorption isotherms of BSA on CaSrHAP particles were pseudo-langmuir type. The saturated amounts of adsorbed BSA (n(s)), which are the plateau values of the adsorption isotherms, linearly increased with the Sr/(Ca+Sr) ratio. Also, n(s) values increased with increasing (Ca+Sr)/P molar ratio, suggesting that the electrostatic attractive force between BSA and CaSrHAP particles is a determining factor of n(s). Despite their large cation/P ratio, however, two particles exhibiting agglomerates with uneven surfaces gave small n(s) values. This fact could be explained by the difference in the particle texture between these two particles and the others; large acicular particles with flat surfaces are advantageous for the adsorption of negatively charged BSA because of the large number of positively charged adsorption sites on the exposed ac or be crystal planes. It was suggested therefore that the texture of the particle is significant in the adsorption of BSA.