Identification of microRNAs by small RNA deep sequencing for synthetic microRNA mimics to control Spodoptera exigua
Identification of microRNAs by small RNA deep sequencing for synthetic microRNA mimics to control Spodoptera exigua
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通过小 RNA 深度测序鉴定 MicroRNA 合成 microRNA 模拟物以控制甜菜夜蛾
DOI：
10.1016/j.gene.2014.12.038
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发表时间：
2015-02-25
期刊：
影响因子：
3.5
通讯作者：
Guo, An Ping
中科院分区：
文献类型：
--
作者：
Zhang, Yu Liang;Huang, Qi Xing;Guo, An Ping
甜菜夜蛾（Spodoptera exigua）是世界各地棉花的主要害虫。随着转基因棉花植物对苏云金芽孢杆菌 (Bt) 毒素的抗性增强，需要开发一种可与 Bt 转基因作物结合使用的替代控制方法，作为抗性管理策略的一部分。 MicroRNA（miRNA）是一个非编码小RNA家族（18-25 nt），在各种生物过程中发挥着至关重要的作用，并且miRNA的过度表达已被证明会干扰昆虫的正常发育。在本研究中，我们利用小RNA深度测序技术鉴定了甜菜夜蛾中127个保守的miRNA。由此，我们测试了 11 种 miRNA 对幼虫发育的影响。我们发现三种 miRNA，Sex-miR-10-1a、SexmiR-4924 和 Sex-miR-9，在甜菜夜蛾幼虫阶段存在差异表达。使用 Sex-miR-1 0-1a、Sex-miR-4924 和 S ex-mi R-9 的合成 miRNA 模拟物进行口服喂养实验，结果抑制了甜菜夜蛾的生长和死亡率。 Sex-miR-4924 的过度表达导致几丁质酶 1 的表达水平显着降低，并导致昆虫蜕皮失败。因此，我们展示了一种使用 miRNA 模拟物来控制甜菜夜蛾发育的新方法。 (C) 2014 Elsevier B.V. 保留所有权利。
Beet armyworm, Spodoptera exigua, is a major pest of cotton around the world. With the increase of resistance to Bacillus thuringiensis (Bt) toxin in transgenic cotton plants, there is a need to develop an alternative control approach that can be used in combination with Bt transgenic crops as part of resistance management strategies. MicroRNAs (miRNAs), a non-coding small RNA family (18-25 nt), play crucial roles in various biological processes and over-expression of miRNAs has been shown to interfere with the normal development of insects. In this study, we identified 127 conserved miRNAs in S. exigua by using small RNA deep sequencing technology. From this, we tested the effects of 11 miRNAs on larval development. We found three miRNAs, Sex-miR-10-1a, SexmiR-4924, and Sex-miR-9, to be differentially expressed during larval stages of S. exigua. Oral feeding experiments using synthetic miRNA mimics of Sex-miR-1 0-1a, Sex-miR-4924, and S ex-mi R-9 resulted in suppressed growth of S. exigua and mortality. Over-expression of Sex-miR-4924 caused a significant reduction in the expression level of chitinase 1 and caused abortive molting in the insects. Therefore, we demonstrated a novel approach of using miRNA mimics to control S. exigua development. (C) 2014 Elsevier B.V. All rights reserved.