Antidromic and orthodromic responses by subicular neurons in rat brain slices.
Antidromic and orthodromic responses by subicular neurons in rat brain slices.
复制标题
大鼠脑切片中皮下神经元的逆向和顺向反应。
DOI：
10.1016/s0006-8993(97)00690-2
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发表时间：
1997
期刊：
影响因子：
2.9
通讯作者：
Stewart,M
中科院分区：
文献类型：
--
作者：
Stewart,M
下托形成海马和内嗅皮层之间过渡区域的一部分，是海马结构的主要输出结构之一。来自皮下破裂和非破裂细胞类型的细胞内记录以及场电位记录是从大鼠大脑的水平切片中获取的。研究两种细胞类型的输入和输出，以加强或反驳基于膜特性提出的二分法。一些爆裂细胞被施加到前下托浅层或深层的刺激逆向激活，但从未被施加到内侧内嗅皮层（dMEC）深层的刺激激活。一些非爆发性的皮下神经元被施加到 dMEC 的刺激逆向激活，但从未被施加到前下层的刺激激活。当刺激深层 MEC 时，下托中的逆向群体事件是单次尖峰，但当刺激前下托时，即使存在谷氨酸受体拮抗剂，也是爆发式的。群体爆发由 2 个或更多群体峰值组成，峰峰值间隔为 ∼5 ms。群体爆发发生在兴奋性传递被阻断的切片中，这表明这种群体爆发反映了单个神经元的同时爆发。两种皮下细胞类型均会响应单一内嗅、皮前和 CA1 刺激而诱发短潜伏期 (<5 ms) 兴奋性突触后电位 (EPSP)。在两种细胞类型中均观察到长潜伏期（>10 ms）EPSP 对皮下刺激作出反应，但对内嗅或 CA1 刺激没有反应。爆发细胞对短暂的顺向刺激（2-10 个脉冲，5-10 毫秒刺激间隔）做出反应，并产生动作电位爆发，即使细胞之前通过电流注入去极化超出了爆发范围。非爆发细胞对短暂的顺向刺激作出反应，在所有保持电位下重复放电（≤1 个尖峰/刺激）。尖峰间隔可以达到通过细胞破裂而出现的突发间隔。结论是：（1）爆发性和非爆发性皮下神经元之间的区别是二分法，细胞在逆向或顺向激活时不会改变其身份； （2）两种细胞类型的输出可能不同：投射到前下托的爆发细胞和投射到内嗅皮层的非爆发细胞； (3)当重复刺激时，非爆发细胞可以发射重复的尖峰，其尖峰间隔在爆发中看到的间隔范围内。
The subiculum forms part of the region of transition between hippocampus and entorhinal cortex and is one of the primary output structures of the hippocampal formation. Intracellular recordings from subicular bursting and non-bursting cell types and field potential recordings were taken in horizontal slices from rat brains. The inputs and outputs of the two cell types were studied for the purpose of reinforcing or refuting the dichotomy proposed on the basis of membrane properties. Some bursting cells were antidromically activated by stimuli applied to the superficial or deep layers of presubiculum, but never by stimuli applied to deep layers of medial entorhinal cortex (dMEC). Some non-bursting subicular neurons were antidromically activated by stimuli applied to dMEC, but never by stimuli applied to presubiculum. Antidromic population events in subiculum were single spikes when deep MEC was stimulated, but were bursts when presubiculum was stimulated, even in the presence of glutamate receptor antagonists. Population bursts consist of 2 or more population spikes with peak to peak intervals of ∼5 ms. That population bursts occur in slices where excitatory transmission is blocked suggests that such population bursts reflect coincident bursts by individual neurons. Short-latency (<5 ms) excitatory postsynaptic potentials (EPSPs) were evoked in both subicular cell types in response to single entorhinal, presubicular and CA1 stimuli. Long-latency (>10 ms) EPSPs were seen in both cell types in response to presubicular, but not entorhinal or CA1 stimulation. Bursting cells responded to brief trains of orthodromic stimuli (2–10 pulses, 5–10 ms interstimulus interval) with a burst of action potentials even when the cell was previously depolarized out of bursting range by current injection. Non-bursting cells responded to brief trains of orthodromic stimuli with repetitive firing (≤1 spike/stimulus) at all holding potentials. Spike intervals could reach those seen in bursts by bursting cells. It is concluded that: (1) the distinction between bursting and non-bursting subicular neurons is a dichotomy and cells do not change their identity when activated antidromically or orthodromically; (2) the outputs of the two cell types may be different: bursting cells projected to presubiculum and non-bursting cells projected to entorhinal cortex; and (3) non-bursting cells can, when repetitively stimulated, fire repetitive spikes with interspike intervals in the range of intervals seen in bursts.