Comprehensive circular RNA profiling of proliferative vitreoretinopathy and its clinical significance.
Comprehensive circular RNA profiling of proliferative vitreoretinopathy and its clinical significance.
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DOI：
10.1016/j.biopha.2018.12.044
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发表时间：
2019-03
期刊：
影响因子：
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通讯作者：
Jin Yao;Lili Hu;Xiu-Miao Li;Kun Shan;Rong-mei Zhou;Hui-min Ge;Mudi Yao;Qin Jiang;Chen Zhao;B. Yan
中科院分区：
文献类型：
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作者：
Jin Yao;Lili Hu;Xiu-Miao Li;Kun Shan;Rong-mei Zhou;Hui-min Ge;Mudi Yao;Qin Jiang;Chen Zhao;B. Yan
增殖性玻璃体视网膜病变（PVR）是视网膜手术的主要挑战之一，多发生于复杂的视网膜手术或穿透性眼损伤患者。环状rna （circRNAs）在许多生物过程和疾病发展中已成为重要的调节因子。然而，circrna在PVR中的特征和功能仍然难以捉摸。在这项研究中，我们在PVR患者的视网膜前膜（ERMs）中发现了91个失调的环状rna。我们进一步研究了circ_0043144的表达模式。circ_0043144在PVR患者的玻璃体样本和相应的血清样本中显著上调。PVR术后circ_0043144表达明显下调。体外研究发现circ_0043144参与了ARPE-19细胞增殖、迁移和分泌能力的调控，对ERM的形成至关重要。总的来说，这项研究表明circrna是PVR发病机制的潜在调节因子。circ_0043144是一种有前景的PVR疾病预后和诊断指标。
Proliferative vitreoretinopathy (PVR) is one of the major challenges in retinal surgery, which occurs in the patient with complex retinal surgery or penetrating eye injury. Circular RNAs (circRNAs) have emerged as important regulators in many biological processes and disease development. However, the characterization and function of circRNAs in PVR remains elusive. In this study, we identified 91 dysregulated circRNAs in the epiretinal membranes (ERMs) of PVR patients. We further investigated the expression pattern of circ_0043144. circ_0043144 was significantly up-regulated in the vitreous samples and the corresponding serum samples of the patients with PVR. circ_0043144 expression was significantly down-regulated after PVR operation. In vitro studies revealed that circ_0043144 was involved in the regulation of the proliferation, migration and secretion ability of ARPE-19 cells, which is critical for ERM formation. Collectively, this study indicates that circRNAs are potential regulators of the pathogenesis of PVR. circ_0043144 is a promising prognostic and diagnostic indicator for PVR diseases.