Phenotypic and genetic characterization of multidrug-resistant Staphylococcus aureus in the tropics of Southeast Asia
Phenotypic and genetic characterization of multidrug-resistant Staphylococcus aureus in the tropics of Southeast Asia
复制标题
东南亚热带地区多重耐药金黄色葡萄球菌的表型和遗传特征
DOI：
10.1099/mic.0.000392
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发表时间：
2016
期刊：
影响因子：
1.5
通讯作者：
Hirofumi Hara
中科院分区：
文献类型：
--
作者：
Siti Norayuni Mohd Zulkeflle;Yus Amira Yusaimi;Norio Sugiura;Koji Iwamoto; Masafumi Goto; Motoo Utsumi;Nor’azizi bin Othman;Zuriati Zakaria;Hirofumi Hara
抗生素耐药性已成为世界各地的主要公共卫生问题。医院废水中存在耐药性细菌如金黄色葡萄球菌和抗生素耐药基因（ARGs）是当今引起极大关注的原因。在这项研究中，276葡萄球菌。在马来西亚，从医院废水样品中回收金酯。所有的菌株进行了筛选敏感性9种不同类别的抗生素：氨苄西林，环丙沙星，庆大霉素，卡那霉素，红霉素，万古霉素，甲氧苄啶和磺胺甲恶唑，氯霉素，四环素和萘啶酸。筛选试验表明，100%的金黄色葡萄球菌对卡那霉素、万古霉素、甲氧苄啶、磺胺甲恶唑和萘啶酸耐药.另外，91%、87%、50%、43%、11%和8.7%的分离株分别对红霉素、庆大霉素、环丙沙星、氨苄青霉素、氯霉素和四环素表现出耐药性。基于这些结果，100%的分离株表现出多药耐药（MDR）特征，对三类以上的抗生素表现出耐药性。在276株分离株中，9株对9种以上的测试抗生素表现出耐药性;选择这些菌株进行抗生素敏感性测试，并检查保守ARG的存在。有趣的是，高百分比的选定金黄色葡萄球菌不含保守的ARG。这些结果表明，非保守的MDR基因元件可能已经扩散到东南亚热带地区的环境中，并且由于全年稳定，温和的温度支持细菌生长，可能已经进化出对几种抗生素的独特抗性机制。
Antibiotic resistance has become a major public health problem throughout the world. The presence of antibiotic-resistant bacteria such asStaphylococcus aureusand antibiotic resistance genes (ARGs) in hospital wastewater is a cause for great concern today. In this study, 276Staph. aureusisolates were recovered from hospital wastewater samples in Malaysia. All of the isolates were screened for susceptibility to nine different classes of antibiotics: ampicillin, ciprofloxacin, gentamicin, kanamycin, erythromycin, vancomycin, trimethoprim and sulfamethoxazole, chloramphenicol, tetracycline and nalidixic acid. Screening tests showed that 100 % ofStaph.aureusisolates exhibited resistance against kanamycin, vancomycin, trimethoprim and sulfamethoxazole and nalidixic acid. Additionally, 91, 87, 50, 43, 11 and 8.7 % of isolates showed resistance against erythromycin, gentamicin, ciprofloxacin, ampicillin, chloramphenicol and tetracycline, respectively. Based on these results, 100 % of isolates demonstrated multidrug-resistant (MDR) characteristics, displaying resistance against more than three classes of antibiotics. Of 276 isolates, nine exhibited resistance to more than nine classes of tested antibiotics; these were selected for antibiotic susceptibility testing and examined for the presence of conserved ARGs. Interestingly, a high percentage of the selected MDRStaph.aureusisolates did not contain conserved ARGs. These results indicate that non-conserved MDR gene elements may have already spread into the environment in the tropics of Southeast Asia, and unique resistance mechanisms against several antibiotics may have evolved due to stable, moderate temperatures that support growth of bacteria throughout the year.