Overlapping divergent transcripts mapping to the HindIII F region of the Autographa californica nuclear polyhedrosis virus.
Overlapping divergent transcripts mapping to the HindIII F region of the Autographa californica nuclear polyhedrosis virus.
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重叠的不同转录本映射到苜蓿银纹夜蛾核多角体病毒的 HindIII F 区域。
DOI：
10.1099/0022-1317-71-1-225
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Weaver,RF
中科院分区：
文献类型：
--
作者：
Hardin,SE;Weaver,RF
我们已经绘制了一组转录本，这些转录本在加利福尼亚核多角体病毒基因组的hindiii F片段中穿过xhoi位点。这些转录本在它们的5 '端重叠了大约550个碱基，并朝相反的方向运行。我们初步确定了两个与左侧转录相对应的开放阅读框。右向转录本在感染后8小时至24小时存在；左侧转录本出现于2 h至24 h。早期（2 h）转录本的大小约为2.1 kb，其5 '端位于xhoi位点右侧约504 bp。从大约8小时开始，一个新的转录起始位点被使用，在第一个位点的下游（向左）约80 bp。晚期（向右）转录本大小约为1.2 kb；它的5 '端似乎是异质的，位于xhoi位点左侧约44至60 bp。在感染后期，通过编码这些转录物的DNA重叠区域，转录同时向两个方向进行。
We have mapped a set of transcripts that cross theXhoI site in theHindIII F fragment of the genome of theAutographa californicanuclear polyhedrosis virus. These transcripts overlap at their 5′ ends by about 550 bases and run in opposite directions. We have tentatively identified two open reading frames corresponding to the leftward transcript. The rightward transcript is present from 8 h post-infection (p.i.) to 24 h p.i.; the leftward transcripts are present from 2 h p.i. to 24 h p.i. The early (2 h) transcript is about 2·1 kb in size and its 5′ end maps about 504 bp to the right of theXhoI site. Beginning at about 8 h p.i. a new transcription start site is used, about 80 bp downstream (to the left) of the first. The late (rightward) transcript is about 1·2 kb in size; its 5′ end seems to be heterogeneous and maps about 44 to 60 bp to the left of theXhoI site. Late in infection transcription proceeds in both directions at the same time through the overlapping region of the DNA encoding these transcripts.