Transcriptomic insight into antimicrobial peptide factors involved in the prophylactic immunity of crowded Mythimna separata larvae
Transcriptomic insight into antimicrobial peptide factors involved in the prophylactic immunity of crowded Mythimna separata larvae
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转录组学研究参与拥挤粘虫幼虫预防性免疫的抗菌肽因子
DOI：
10.1016/j.dci.2019.02.009
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发表时间：
2019-09-01
影响因子：
2.9
通讯作者：
Zhu, Shude
中科院分区：
文献类型：
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作者：
Kong, Hailong;Dong, Chuanlei;Zhu, Shude
与病原性感染相似，高种群密度改变了昆虫的预防性免疫。抗微生物肽（Antimicrobial Peptides，AMP）在昆虫对微生物感染的体液免疫应答中起着重要作用。我们应用RNA测序研究脂肪体和血细胞样本中的差异基因表达水平从幼虫饲养在高密度（10幼虫每罐）和低密度（1幼虫每罐）的条件下，样品表现出密度依赖性的预防。首次从145，439个来自M.分离幼虫随着高密度幼虫的预防性免疫，格洛弗林、防御素、抗菌肽、lebocin和attacin相关单基因等AMP分子的转录水平增加。模式识别受体肽聚糖识别蛋白（PGRP），在IMD和Toll通路相关的unigenes的AMP合成的关键蛋白质，也上调的幼虫从高密度组。所得到的转录组数据库进行了验证的转录水平的四个选定的AMP基因定量从高密度和低密度幼虫组与定量实时PCR。高密度组幼虫血淋巴对革兰氏阳性金黄色葡萄球菌、枯草芽孢杆菌、革兰氏阴性爱德华氏菌和鳗弧菌的抗菌活性显著高于低密度组。我们的研究结果首次揭示了AMP基因在M. separata的研究，为进一步研究该菌的防治提供了新的思路。生物农药的分离。
Similar to pathogenic infection, a high population density alters insect prophylactic immunity. Antimicrobial peptides (AMPs) are known to play critical roles in an insect's humoral immune response to microbial infection. We applied RNA sequencing to investigate differential gene expression levels in fat body and hemocyte samples from larvae reared in high-(10 larvae per jar) and low-density (1 larva per jar) conditions; the samples exhibited density-dependent prophylaxis. A number of AMP molecule-related proteins were annotated for the first time from 145,439 assembled unigenes from M. separata larvae. The transcript levels of AMP molecules such as gloverin-, defensin-, cecropin-, lebocin- and attacin-related unigenes were increased with the prophylactic immunity of high-density larvae. The pattern recognition receptor peptidoglycan recognition protein (PGRP), a key protein in the synthesis of AMPs in IMD- and Toll pathway-related unigenes, was also upregulated in the larvae from the high-density group. The resultant transcriptomic database was validated by the transcript levels of four selected AMP genes quantified from the high- and low-density larval groups with quantitative real-time PCR. The antimicrobial activity against gram-positive Staphylococcus aureus and Bacillus subtilis and gram-negative Edwardsiella ictaluri and Vibrio anguillarum in the hemolymph of larvae from the high-density group was significantly higher than that of larvae from the low-density group. Our findings provide the first insight into the role of AMP genes in the mechanisms of density-dependent prophylaxis in M. separata and provide new insight into the control of M. separata with biopesticides.