Histone Deacetylase HDA9 With ABI4 Contributes to Abscisic Acid Homeostasis in Drought Stress Response
Histone Deacetylase HDA9 With ABI4 Contributes to Abscisic Acid Homeostasis in Drought Stress Response
复制标题
组蛋白脱乙酰酶HDA9和ABI4参与干旱胁迫反应中的脱落酸稳态
DOI：
10.3389/fpls.2020.00143
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发表时间：
2020-02-25
影响因子：
5.6
通讯作者：
Yun, Dae-Jin
中科院分区：
文献类型：
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作者：
Baek, Dongwon;Shin, Gilok;Yun, Dae-Jin
干旱胁迫是影响植物生长和繁殖的重要环境因子。植物已经进化出复杂的分子机制来耐受干旱胁迫。在这项研究中，我们研究了拟南芥RPD 3型组蛋白去乙酰化酶9（HDA 9）在干旱胁迫下的功能。功能缺失突变体hda 9 -1和hda 9 -2对脱落酸（阿坝）不敏感，对干旱胁迫敏感。hda 9 -1突变体中的阿坝含量在野生型（WT）植物中降低。动物和植物中的大多数组蛋白脱乙酰酶与其他染色质重塑组分（如转录因子）形成复合物。在这项研究中，我们发现HDA 9相互作用的阿坝不敏感4（ABI 4）转录因子使用酵母双杂交试验和免疫共沉淀。干旱胁迫诱导hda 9 -1、hda 9 -2、abi 4和hda 9 -1 abi 4突变体中编码阿坝分解代谢途径关键酶（+）-阿坝8 '-羟化酶的CYP 707 A1和CYP 707 A2的表达。染色质免疫沉淀和定量PCR结果表明，HDA 9和ABI 4复合物抑制CYP 707 A1和CYP 707 A2的表达，直接结合到他们的启动子响应干旱胁迫。综上所述，这些数据表明HDA 9和ABI 4形成抑制复合物来调节CYP 707 A1和CYP 707 A2在拟南芥中响应干旱胁迫的表达。
Drought stress, a major environmental factor, significantly affects plant growth and reproduction. Plants have evolved complex molecular mechanisms to tolerate drought stress. In this study, we investigated the function of the Arabidopsis thaliana RPD3-type HISTONE DEACETYLASE 9 (HDA9) in response to drought stress. The loss-of-function mutants hda9-1 and hda9-2 were insensitive to abscisic acid (ABA) and sensitive to drought stress. The ABA content in the hda9-1 mutant was reduced in wild type (WT) plant. Most histone deacetylases in animals and plants form complexes with other chromatin-remodeling components, such as transcription factors. In this study, we found that HDA9 interacts with the ABA INSENSITIVE 4 (ABI4) transcription factor using a yeast two-hybrid assay and coimmunoprecipitation. The expression of CYP707A1 and CYP707A2, which encode (+)-ABA 8 '-hydroxylases, key enzymes in ABA catabolic pathways, was highly induced in hda9-1, hda9-2, abi4, and hda9-1 abi4 mutants upon drought stress. Chromatin immunoprecipitation and quantitative PCR showed that the HDA9 and ABI4 complex repressed the expression of CYP707A1 and CYP707A2 by directly binding to their promoters in response to drought stress. Taken together, these data suggest that HDA9 and ABI4 form a repressive complex to regulate the expression of CYP707A1 and CYP707A2 in response to drought stress in Arabidopsis.