Genome sequence and organization analysis of Heliothis virescens ascovirus 3f isolated from a Helicoverpa zea larva
Genome sequence and organization analysis of Heliothis virescens ascovirus 3f isolated from a Helicoverpa zea larva
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从玉米夜蛾幼虫中分离的烟夜蛾子囊病毒 3f 的基因组序列和组织分析
DOI：
10.1016/j.jip.2014.08.003
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发表时间：
2014
影响因子：
3.4
通讯作者：
Huang Guo-Hua
中科院分区：
文献类型：
--
作者：
Wei Yong-Lu;Hu Jue;Li Shun-Ji;Chen Zi-Shu;Cheng Xiao-Wen;Huang Guo-Hua
获得了烟夜蛾病毒3f（Heliothisvirescensascovirus 3f，HvAV-3f）的全基因组序列。HvAV-3f基因组具有198，157 bp的环状基因组，G + C含量为46.0%，并且编码190个大于69个氨基酸的开放阅读框（ORF）。在基因组中发现了两个主要的同源区（hrs）和29个杆状病毒重复序列（bro）。BLAST分析表明，其中3个HvAV-3f基因与鳞翅目昆虫同源。9个ORF是HvAV-3f所特有的，其中2个ORF与Genbank数据库中先前未描述的囊病毒基因具有显著的相似性。
The complete genome sequence ofHeliothis virescensascovirus 3f (HvAV-3f) was obtained. The HvAV-3f genome has a circular genome of 198,157 bp with a G + C content of 46.0%, and encodes 190 open reading frames (ORFs) longer than 69 amino acids. Two major homologous regions (hrs) and 29 ‘baculovirus repeat ORFs’ (bro) were found in the genome. BLAST analyses revealed that three HvAV-3f genes were homologous to that of lepidopteran insects. Nine ORFs were unique to HvAV-3f, in which two ORFs showed significant levels of similarity to genes that have not been previously described for ascoviruses in the Genbank database.