Immobilization of Candida antarctic lipase B on MWNTs modified by ionic liquids with different functional groups.
Immobilization of Candida antarctic lipase B on MWNTs modified by ionic liquids with different functional groups.
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DOI：
10.1016/j.colsurfb.2017.09.037
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发表时间：
2017-12
期刊：
影响因子：
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通讯作者：
Xiaomei Wan;Xinran Xiang;Susu Tang;Dinghua Yu;He Huang;Y. Hu
中科院分区：
文献类型：
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作者：
Xiaomei Wan;Xinran Xiang;Susu Tang;Dinghua Yu;He Huang;Y. Hu
采用具有烷基、羧基、氨基和羟基等不同官能团的咪唑类离子液体对多壁碳纳米管（MWNTs）进行修饰。将该载体用于固定化厌氧假丝酵母脂肪酶B（CALB），以三乙酸甘油酯为底物，研究了不同官能团离子液体对CALB酶性质的影响。结果表明，功能化过程没有破坏碳纳米管的结构完整性，不同功能基团的离子液体修饰碳纳米管后，固定化CALB的酶学性能均得到提高。选择具有最佳酶促性能的羟基功能化离子液体，考察了不同碳链长度对固定化CALB酶促性能的影响。其中，以合适链长的羟基功能化离子液体修饰的MWNTs（MWNTs-IL（8 C）-OH-CALB）为载体固定化的CALB比活性最高，是MWNTs-CALB的18.11倍。此外，它还表现出最好的热稳定性和可重复使用性。MWNTs-IL（8 C）-OH-CALB在70 ℃下孵育20 min后，剩余活性仍为初始活性的64.01%，使用4次后剩余活性为85.56%。
Multiwalled carbon nanotubes (MWNTs) were modified by imidazole-based ionic liquids with different kinds of functional groups such as alkyl, carboxyl, amino and hydroxyl. The supports were used to immobilizeCandida antarcticlipase B (CALB) and the influence of different functional groups of ionic liquids on enzymatic properties was investigated by the hydrolysis reaction of triacetin. The results revealed that the functionalization process did not destroy the structural integrity of MWNTs, and the enzymatic properities of CALB which immobilized on the MWNTs modified by ionic liquids with different kinds of functional groups were all improved. The hydroxyl functionalized ionic liquids which exhibited the best enzymatic properities was selected to investigate the effects of different carbon chain length on the enzymatic properties of immobilized CALB. Among them, CALB which immobilized on MWNTs modified by hydroxyl functionalized ionic liquid with suitable chain length (MWNTs-IL(8C)-OH-CALB) had the highest specific activity, with a specific activity of 18.11 times that of MWNTs-CALB. Furthermore, it also presented best thermal stability and reusability. The residual activity of MWNTs-IL(8C)-OH-CALB held over 64.01% of the initial activity after being incubated for 20 min at 70 °C, and the residual activity was 85.56% after 4 cycles of use.