Lensless imaging for simultaneous microfluidic sperm monitoring and sorting.
Lensless imaging for simultaneous microfluidic sperm monitoring and sorting.
复制标题
用于同步微流控精子监测和分选的无透镜成像。
DOI：
10.1039/c1lc20236g
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发表时间：
2011
期刊：
影响因子：
6.1
通讯作者：
Demirci,Utkan
中科院分区：
文献类型：
--
作者：
Zhang,Xiaohui;Khimji,Imran;Gurkan,UmutAtakan;Safaee,Hooman;Catalano,PaoloNicolas;Keles,HasanOnur;Kayaalp,Emre;Demirci,Utkan
5.3在美国，约有100万对育龄夫妇（9%）受到不孕症的影响，其中男性因素占50%，这就需要确定定义精子质量的参数，包括精子计数和活力。体外受精（IVF）结合或不结合胞质内单精子注射（ICSI）已成为现代临床实践中最广泛使用的辅助生殖技术（ART），以克服男性不育的挑战。IVF和ICSI的障碍之一在于从具有低精子计数（少精子症）和/或低精子活力（少精子症）的精液样本中识别和分离最活跃和可能最健康的精子。微流体系统已经显示出利用流动系统分选精子的潜力。然而，通常用于对精子运动进行成像的常规显微镜的小视场（FOV）在同时跟踪大量精子细胞方面提出了挑战。为了应对这一挑战，我们将无透镜电荷耦合器件（CCD）与微流控芯片集成在一起，以便在精子在微流控通道内移动时实现宽FOV和自动记录。该集成系统能够分选和跟踪已放置在微流体通道中的精子群体。该通道可以在水平和垂直配置下进行监测，类似于临床使用的上游柱方法。精子运动性可以通过追踪单个精子的阴影路径来量化。此外，由于精子通过从微流体通道的入口向出口游动而被分选，因此到达出口的活动精子可以在过程结束时从通道中提取。该技术可以导致在广泛的视野中根据运动反应单独评估每个精子的方法，这可以证明在处理少精子症或少精子症样品时特别有用，其中最活跃的精子需要从少量精子的池中分离出来。
5.3 million American couples of reproductive age (9%) are affected by infertility, among which male factors account for up to 50% of cases, which necessitates the identification of parameters defining sperm quality, including sperm count and motility. In vitrofertilization (IVF) with or without intra cytoplasmic sperm injection (ICSI) has become the most widely used assisted reproductive technology (ART) in modern clinical practice to overcome male infertility challenges. One of the obstacles of IVF and ICSI lies in identifying and isolating the most motile and presumably healthiest sperm from semen samples that have low sperm counts (oligozoospermia) and/or low sperm motility (oligospermaesthenia). Microfluidic systems have shown potential to sort sperm with flow systems. However, the small field of view (FOV) of conventional microscopes commonly used to image sperm motion presents challenges in tracking a large number of sperm cells simultaneously. To address this challenge, we have integrated a lensless charge-coupled device (CCD) with a microfluidic chip to enable wide FOV and automatic recording as the sperm move inside a microfluidic channel. The integrated system enables the sorting and tracking of a population of sperm that have been placed in a microfluidic channel. This channel can be monitored in both horizontal and vertical configuration similar to a swim-up column method used clinically. Sperm motilities can be quantified by tracing the shadow paths for individual sperm. Moreover, as the sperm are sorted by swimming from the inlet towards the outlet of a microfluidic channel, motile sperm that reach the outlet can be extracted from the channel at the end of the process. This technology can lead to methods to evaluate each sperm individually in terms of motility response in a wide field of view, which could prove especially useful, when working with oligozoospermic or oligospermaesthenic samples, in which the most motile sperm need to be isolated from a pool of small number of sperm.