Tertiary Lymphoid Organs in Lymphatic Malformations
Tertiary Lymphoid Organs in Lymphatic Malformations
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DOI：
10.1089/lrb.2010.0018
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发表时间：
2011-01-01
影响因子：
1.4
通讯作者：
Perkins, Jonathan A.
中科院分区：
文献类型：
--
作者：
Kirsh, Andrew L.;Cushing, Sharon L.;Perkins, Jonathan A.
背景：检查淋巴畸形淋巴聚集体中三级淋巴器官标志物的表达。确定淋巴聚集密度与淋巴畸形临床特征的关系。方法和结果：回顾性队列研究。回顾了血管异常数据库中 29 名患者的组织和临床数据，这些患者代表了头颈部淋巴管畸形的范围，并且随访时间超过 5 年。存档的福尔马林固定、石蜡包埋的淋巴畸形组织用三级淋巴器官标志物的抗体进行免疫组织化学染色，其中包括滤泡和成熟的骨髓树突状细胞、高内皮小静脉、分离的 B 和 T 细胞、淋巴内皮细胞和淋巴归巢趋化因子（CXCL13、CCL21）。淋巴聚集密度（计数/mm(2)）由 2 名独立、盲法评审员量化。使用方差分析来表征淋巴聚集密度和淋巴畸形临床特征。较大的淋巴畸形组织淋巴聚集体的三级淋巴器官标记物染色一致。在同一患者的口腔和颈部标本中（n = 9），口腔中的三级淋巴器官比颈部标本中更多（p = 0.0235）。在淋巴管畸形颈部组织中，de Serres 4 期淋巴管畸形显示出最高的三级淋巴器官密度。三级淋巴器官密度与其他临床特征之间没有发现显着关联。结论：本研究表明，淋巴畸形内的一些淋巴聚集体代表三级淋巴器官。三级淋巴器官密度与淋巴畸形位置之间存在关联。需要进一步研究来明确淋巴新生和三级淋巴器官形成在淋巴畸形发病机制中的作用。
Background: Examine lymphatic malformation lymphoid aggregates for the expression of tertiary lymphoid organ markers. Determine how lymphoid aggregate density relates to lymphatic malformation clinical features.Methods and Results: Retrospective cohort study. Tissue and clinical data were reviewed from 29 patients in the Vascular Anomaly Database who represented the spectrum of head and neck lymphatic malformations and had >5 years of follow-up. Archived formalin-fixed, paraffin-embedded lymphatic malformation tissue was immunohistochemically stained with antibodies for tertiary lymphoid organ markers, which included follicular and mature myeloid dendritic cells, high endothelial venules, segregated B and T-cells, lymphatic endothelial cells, and lymphoid homing chemokines (CXCL13, CCL21). Lymphoid aggregate density (count/mm(2)) was quantified by 2 independent, blinded reviewers. Lymphoid aggregate density and lymphatic malformation clinical features were characterized using analysis of variance. Larger lymphatic malformation tissue lymphoid aggregates stained consistently for tertiary lymphoid organ markers. In oral cavity and neck specimens from the same patients (n = 9), there were more tertiary lymphoid organ in oral cavity than in neck specimens (p = 0.0235). In lymphatic malformation neck tissue, de Serres stage 4 lymphatic malformations displayed the highest tertiary lymphoid organ density. No significant association was seen between tertiary lymphoid organ density and other clinical features.Conclusion: This study demonstrates that some lymphoid aggregates within lymphatic malformations represent tertiary lymphoid organs. There was an association between tertiary lymphoid organ density and lymphatic malformation location. Further study is required to define the role of lymphoid neogenesis and tertiary lymphoid organ formation in lymphatic malformation pathogenesis.