Biochar facilitates rapid restoration of methanogenesis by enhancing direct interspecies electron transfer after high organic loading shock
Biochar facilitates rapid restoration of methanogenesis by enhancing direct interspecies electron transfer after high organic loading shock
复制标题
生物炭通过增强高有机负荷冲击后的直接种间电子转移来促进产甲烷作用的快速恢复
DOI：
10.1016/j.biortech.2020.124360
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发表时间：
2021
影响因子：
11.4
通讯作者：
Liang Zhu
中科院分区：
文献类型：
--
作者：
Chen Wang;Yang Liu;Caiqin Wang;Bo Xing;Shaodong Zhu;Jinjing Huang;Xiangyang Xu;Liang Zhu
本研究评估了生物炭添加对上流式厌氧污泥床（UASB）反应器（R1与生物炭; R2无生物炭）中80 kg COD/m3/d的高有机负荷冲击（OLS）的有效性。经过24 h的OLS后，R2出现不可逆酸化（pH为5.42 ± 0.07），产气量较低，为0.08 ± 0.01 m3/kg COD/d。相反，在R1中的沼气产量迅速恢复到0.33 ± 0.04 m3/kg COD/d，并且在R1中的出水pH恢复到7.01 ± 0.22。添加生物炭后，R1中富集了潜在的直接种间电子传递（DIET）伙伴，包括挥发性脂肪酸（VFAs）氧化细菌（拟杆菌属、Smithella、脱硫弧菌属、Geolecum）和产甲烷菌（甲烷八叠球菌属、甲烷菌属），有利于维持产酸产甲烷平衡。此外，生物炭对甲烷菌和甲烷菌的截留作用，维持了R1中颗粒污泥在OLS和VFAs压力下的结构稳定性，保证了厌氧系统的稳定性。
This study evaluated the effectiveness of biochar addition against high organic loading shock (OLS) of 80 kg COD/m3/d in up-flow anaerobic sludge blanket (UASB) reactors (R1 with biochar; R2 without biochar). After OLS of 24 h, R2 suffered the irreversible acidification (pH of 5.42 ± 0.07) with low biogas production of 0.08 ± 0.01 m3/kg COD/d. In contrast, the biogas production in R1 restored rapidly to 0.33 ± 0.04 m3/kg COD/d, and effluent pH in R1 returned to 7.01 ± 0.22. With addition of biochar, potential direct interspecies electron transfer (DIET) partners, including volatile fatty acids (VFAs)-oxidizing bacteria (Bacteroidetes,Smithella,Desulfovibrio,Geobacter) and methanogens (Methanosaeta,Methanosarcina) were enriched in R1, which were conductive to maintain the balance of acidogenesis and methanogenesis. Moreover, the retention ofMethanosaetaandMethanosarcinacoupled with biochar maintained the structural stability of granular sludge in R1 under the pressure of OLS and VFAs, which guaranteed the stability of anaerobic system.