Enhanced Mass Defect Filtering To Simplify and Classify Complex Mixtures of Lignin Degradation Products
Enhanced Mass Defect Filtering To Simplify and Classify Complex Mixtures of Lignin Degradation Products
复制标题
强化质量亏损过滤：简化木质素降解产物复杂混合物并进行分类
DOI：
10.1021/acs.analchem.5b03790
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发表时间：
2016-01-19
影响因子：
7.4
通讯作者：
Volmer, Dietrich A.
中科院分区：
文献类型：
--
作者：
Dier, Tobias K. F.;Egele, Kerstin;Volmer, Dietrich A.
利用高分辨质谱研究了木质素电化学分解产生的高度复杂的产物混合物。由于大多数降解产物的化学结构是未知的，增强的质量缺陷过滤技术的实施，以简化表征的混合物。结果表明，实施的电离技术有重大影响的范围内可检测的击穿产品，与大气压光电离负电离模式提供了最广泛的覆盖范围在我们的实验。使用不同的修改的肯德里克质量图作为质量缺陷过滤的基础，其中利用肯德里克质量缺陷和木质素特异性愈创木酚（C7H7O2）单体单元的质量缺陷，容易地允许独立于产物的低聚状态的类别分配。因此，增强的质量缺陷过滤策略提供了样品组成的快速表征。此外，通过与该化合物系列的初步鉴别产物进行比较，确定了降解序列中化合物之间的结构相似性。在一般情况下，我们的分析表明，主要分解产物与低氧含量下形成的电化学条件下使用质子离子液体作为溶剂的木质素。
High resolution mass spectrometry was utilized to study the highly complex product mixtures resulting from electrochemical breakdown of lignin. As most of the chemical structures of the degradation products were unknown, enhanced mass defect filtering techniques were implemented to simplify the characterization of the mixtures. It was shown that the implemented ionization techniques had a major impact on the range of detectable breakdown products, with atmospheric pressure photoionization in negative ionization mode providing the widest coverage in our experiments. Different modified Kendrick mass plots were used as a basis for mass defect filtering, where Kendrick mass defect and the mass defect of the lignin-specific guaiacol (C7H7O2) monomeric unit were utilized, readily allowing class assignments independent of the oligomeric state of the product. The enhanced mass defect filtering strategy therefore provided rapid characterization of the sample composition. In addition, the structural similarities between the compounds within a degradation sequence were determined by comparison to a tentatively identified product of this compound series. In general, our analyses revealed that primarily breakdown products with low oxygen content were formed under electrochemical conditions using protic ionic liquids as solvent for lignin.