Evaluation of a novel fluorescent nanobeacon for targeted imaging of Thomsen-Friedenreich associated colorectal cancer.
Evaluation of a novel fluorescent nanobeacon for targeted imaging of Thomsen-Friedenreich associated colorectal cancer.
复制标题
DOI：
10.2147/ijn.s124174
复制
发表时间：
2017
影响因子：
8
通讯作者：
Pham W
中科院分区：
文献类型：
--
作者：
Nakase H;Sakuma S;Fukuchi T;Yoshino T;Mohri K;Miyata K;Kumagai H;Hiwatari KI;Tsubaki K;Ikejima T;Tobita E;Zhu M;Wilson KJ;Washington K;Gore JC;Pham W
Thomsen-Friedenreich(TF)抗原是判断结直肠癌预后的生物标志物。在这里，我们使用表面以花生凝集素作为TF结合分子的纳米生物素，证明了该纳米生物素能够在荧光显微镜下检测冷冻和新鲜活检的息肉中的TF抗原。我们的结果为如何以最及时的方式检测异常结肠组织提供了重要线索。考虑到这种局部纳米离子的通用应用方法，这项工作中使用的方案适用于临床结肠镜检查。此外，这项技术的临床翻译前景是显而易见的。
The Thomsen–Friedenreich (TF) antigen represents a prognostic biomarker of colorectal carcinoma. Here, using a nanobeacon, the surface of which was fabricated with peanut agglutinin as TF-binding molecules, we demonstrate that the nanobeacon is able to detect TF antigen in frozen and freshly biopsied polyps using fluorescence microscopy. Our results provide important clues about how to detect aberrant colonic tissues in the most timely fashion. Given the versatile application method for this topical nanobeacon, the protocol used in this work is amenable to clinical colonoscopy. Moreover, the prospects of clinical translation of this technology are evident.