Simultaneous measurements of Cr, Cd, Hg and Pb species in ng L-1 levels by interfacing high performance liquid chromatography and inductively coupled plasma mass spectrometry
Simultaneous measurements of Cr, Cd, Hg and Pb species in ng L-1 levels by interfacing high performance liquid chromatography and inductively coupled plasma mass spectrometry
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DOI：
10.1016/j.aca.2022.339935
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发表时间：
2022-05-18
影响因子：
6.2
通讯作者：
Cheng, Heyong
中科院分区：
文献类型：
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作者：
Song, Yihuan;Guo, Feng;Cheng, Heyong
铬、镉、汞和铅的毒性、迁移性、生物利用度和生物功能在很大程度上取决于其特定的化学形态，导致对金属形态分析的需求很高，而不是总量定量。多金属（类金属）的同时形态分析由于其相对于传统的单一元素策略的经济和环境优势而对含有不稳定分析物的大样品容量具有吸引力。在这项工作中，一种分析方法集成在线固相萃取到高效液相色谱接口与电感耦合等离子体质谱（ICP-MS），同时预浓缩和定量铬，镉，汞和铅的形式在pg L-1水平的水开发。CR C18吸附剂对重金属（III + VI）、Cd（II）、Hg（II）、Pb（II）、甲基汞（MeHg）、乙基汞（EtHg）、三甲基铅（TML）和三乙基铅（TEL（用10 mL 1.0 mM 2-羟基乙烷以10 mL min-1平衡），并通过移动的流动相洗脱0），然后在8.0分钟内在C18柱上完全分离，并最终通过ICP-MS测定。0.001-0.007 ng L-1）和定量（0.003-0.023 ng L-1），良好的相对标准偏差（
Toxicity, mobility, bioavailability and biofunctions of chromium, cadmium, mercury and lead are heavily dependent upon their specific chemical forms, leading to a high demand to metal speciation analysis rather than total quantification. Simultaneous speciation analysis of multiple metal(loid)s is attractive to a large sample capacity containing unstable analytes due to its economic and environmental advantages over the conventional single elemental strategies. In this work, an analytical method integrating online solid phase extraction into high performance liquid chromatography interfaced with inductively coupled plasma mass spectrometry (ICP-MS) to simultaneously preconcentrate and quantify Cr, Cd, Hg and Pb forms in pg L-1 levels in water was developed. Cr (III + VI), Cd(II), Hg(II), Pb(II), methylmercury (MeHg), ethylmercury (EtHg), and trimethyl (TML) and triethyl lead (TEL) were captured by the C18 adsorbent (equilibrated with 10 mL of 1.0 mM 2-hydroxyethanethiol at 10 mL min-1) and eluted by mobile phase (5.0 mM Cys at pH 2.0), then completely separated on the C18 column within 8.0 min and eventually determined by ICP-MS. Low limits of detection (0.001-0.007 ng L-1) and quantification (0.003-0.023 ng L-1), good relative standard deviations (