Myoglobin immunoassay based on metal particle-enhanced fluorescence
Myoglobin immunoassay based on metal particle-enhanced fluorescence
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DOI：
10.1016/j.jim.2005.04.020
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发表时间：
2005-07-01
影响因子：
2.2
通讯作者：
Lakowicz, JR
中科院分区：
文献类型：
--
作者：
Matveeva, EG;Gryczynski, Z;Lakowicz, JR
利用银岛膜 (SIF) 上的增强荧光来开发心脏标志物肌红蛋白 (Myo) 的夹心式免疫测定法。肌红蛋白首先被捕获在涂有抗 Myo 抗体的表面上；然后将表面与荧光标记的抗 Myo 抗体一起孵育。该系统在玻璃表面和 SIF 上进行了检查。我们观察到，与未使用 SIR A 动力学免疫测定法对 SIF 修饰表面上的 Myo 修饰的玻璃基板发出的信号相比，SIF 信号增强了 10-15 倍，导致背景信号降低。初步结果表明，可以检测到低于 50 ng/mL 的肌红蛋白浓度，该浓度低于健康患者肌红蛋白的临床临界值。我们建议使用 SIF 修饰的底物来提高荧光检测表面测定的灵敏度。 (c) 2005 Elsevier B.V. 保留所有权利。
Enhanced fluorescence on silver island films (SIFs) is utilized to develop a sandwich-format immunoassay for the cardiac marker myoglobin (Myo). Myoglobin was first captured on surfaces coated with anti-Myo antibodies; the surface was then incubated with fluorescently labeled anti-Myo antibodies. The system was examined on glass surfaces and on SIFs. We observed the enhancement of the signal from SIFs in the range of 10-15-fold if compared to the signal from the glass substrate not modified with a SIR A kinetic immunoassay for Myo on SIF-modified surface results in a decreased background signal. The initial results show that it is possible to detect Myoglobin concentrations below 50 ng/mL, which is lower than clinical cut-off for Myoglobin in healthy patients. We suggest the use of SIF-modified substrates for increasing the sensitivity of surface assays with fluorescence detection. (c) 2005 Elsevier B.V. All rights reserved.