ULTRASTRUCTURE OF EPIDERMIS OF MICE WITH CHRONIC PROLIFERATIVE DERMATITIS
ULTRASTRUCTURE OF EPIDERMIS OF MICE WITH CHRONIC PROLIFERATIVE DERMATITIS
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DOI：
10.3109/01913129509014610
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发表时间：
1995-03-01
影响因子：
1
通讯作者：
ZURCHER, C
中科院分区：
文献类型：
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作者：
GIJBELS, MJJ;HOGENESCH, H;ZURCHER, C
慢性增生性皮炎（cpdm/cpdm）的C57BL/Ka小鼠发生慢性持续性皮肤病变，其特征为表皮增生、粒细胞和巨噬细胞浸润、血管扩张。类似的病变也存在于受影响小鼠的其他正角化上皮中，特别是食道和前胃。在这里，我们报告进一步表征表皮增生和粒细胞。病变皮肤的角质形成细胞，而不是正常皮肤的角质形成细胞，在表皮的所有层中都有圆形和电子密集的线粒体包涵体。在受感染小鼠的食道和前胃中也存在类似的内含物。在小鼠中，线粒体内包涵体的存在与表皮增生似乎有直接关系。此外，在病变中经常观察到表皮、食道和前胃中存在角化细胞衍生的凋亡小体，这与先前光镜下观察到的角化细胞单细胞死亡相一致。皮肤、食道和前胃的粒细胞以嗜酸性粒细胞为主。在表皮致密层中可见广泛的间隙，这些间隙主要与真皮或表皮嗜酸性粒细胞直接相关。在人类牛皮癣样疾病中也观察到这种类型的间隙。电镜研究表明，该小鼠模型可用于筛选银屑病和其他炎症性皮肤病的潜在治疗策略。
C57BL/Ka mice with chronic proliferative dermatitis (cpdm/cpdm) develop chronic persistent skin lesions characterized by epidermal hyperplasia, infiltration by granulocytes and macrophages, and vascular dilatation. Similar lesions are present in other orthokeratotic epithelia in affected mice, in particular the esophagus and forestomach. Here, we report on further characterization of epidermal hyperplasia and the granulocytes. Keratinocytes of lesional skin, but not of normal skin, show round and electron-dense mitochondrial inclusions that are present in all layers of the epidermis. Similar inclusions are also present in the esophagus and forestomach of affected mice. There appears to be a direct relation between the presence of intramitochondrial inclusions and epidermal hyperplasia in the mouse. Furthermore, the presence of keratinocyte-derived apoptotic bodies in the epidermis, esophagus, and forestomach was frequently observed in the lesions, which is consistent with previous light microscopic observations of single cell death of keratinocytes. The granulocytes present in the skin, esophagus, and forestomach were mainly eosinophils. There were widespread gaps observed in the lamina densa in the epidermis that were mostly directly associated with dermal or epidermal eosinophils. This type of gap is also observed in psoriasiform diseases in humans. This electron microscopic study demonstrated that this mouse model should be useful to screen potential therapeutic strategies for psoriasiform and other inflammatory skin disorders.