Age-dependent organotypic expression of microtubule-associated proteins (MAP1, MAP2, and MAP5) in rat brain
Age-dependent organotypic expression of microtubule-associated proteins (MAP1, MAP2, and MAP5) in rat brain
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DOI：
10.1023/a:1027306227402
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发表时间：
1997-06-01
影响因子：
4.4
通讯作者：
Siegel, G
中科院分区：
文献类型：
--
作者：
Chauhan, N;Siegel, G
分析了幼龄（3月龄，N = 3）和老年（24月龄，N = 3）大鼠脑中微管相关蛋白（MAPs）分布的年龄依赖性变化。在幼龄大鼠中，MAP1和MAPS在核周和树突中表现出明显的免疫染色，而MAP2则选择性地定位于树突。在小脑中，MAP2优先定位于浦肯野细胞树突的细分支和远分支以及肾小球周围的点状沉积物。总体而言，衰老导致海马和顶叶皮层MAP2- >>、MAP1-和maps免疫反应活性明显下降，小脑无变化。结果表明：(1)老年大鼠脑map -免疫反应性下降以海马区受影响最大，小脑受影响最小；(2)在海马和皮层的大多数树突中，树突特异性MAP2几乎完全被耗尽。综上所述，受影响脑区map2免疫反应性的丧失可能与突触可塑性、认知和记忆功能的年龄相关损伤有关。
Age-dependent changes in the distribution of microtubule-associated proteins (MAPs) were analyzed in young (3-months, N = 3) and old (24-months, N = 3) rat brain. In the young rats, MAP1 and MAPS exhibited prominent immunostaining in the perikarya and dendrites whereas MAP2 was selectively localized in the dendrites. In the cerebellum, MAP2 was preferentially localized in finer and distal branches of Purkinje cell dendrites and in punctate deposits surrounding glomeruli. In general, aging resulted in obvious declines in MAP2- >> MAP1- and MAPS-immunoreactivities in the hippocampus and parietal cortex but no change in cerebellum. The results indicate that: (1) hippocampus is the most affected and cerebellum is the least affected region with regard to declines in MAPs-immunoreactivities in the aged rat brain; (2) dendrite-specific MAP2 is almost completely depleted from most dendrites in the hippocampus and cortex. In summary, loss of MAP2-immunoreactivity in the affected brain areas may be associated with age-related impairment of synaptic plasticity, cognition and memory functions.