Protective Effect of L-Theanine on Cadmium-Induced Apoptosis in PC12 Cells by Inhibiting the Mitochondria-Mediated Pathway
Protective Effect of L-Theanine on Cadmium-Induced Apoptosis in PC12 Cells by Inhibiting the Mitochondria-Mediated Pathway
复制标题
L-茶氨酸通过抑制线粒体介导的途径对镉诱导的 PC12 细胞凋亡的保护作用
DOI：
10.1007/s11064-015-1648-4
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发表时间：
2015-08-01
影响因子：
4.4
通讯作者：
Luo, Lan
中科院分区：
文献类型：
--
作者：
Ben, Peiling;Zhang, Zhengping;Luo, Lan
L-茶氨酸是从绿色茶中提取的一种氨基酸衍生物。本工作旨在研究L-茶氨酸对氯化镉刺激的神经元样大鼠嗜铬细胞瘤（PC 12）细胞的影响。在镉暴露前用l-茶氨酸处理可增加细胞活力; Annexin V/PI染色实验表明l-茶氨酸可抑制镉诱导的细胞凋亡。同时，L-茶氨酸减少ROS的产生，并保护镉诱导的线粒体跨膜电位的破坏。与镉处理的细胞相比，L-茶氨酸还可降低Bax/Bcl-2比值，以及caspase-9、caspase-3和poly（ADP-核糖）聚合酶的水平。此外，l-茶氨酸抑制镉诱导的PI 3 K/Akt、MAPK ERK 1/2和JNK信号转导的磷酸化上调。这些数据表明，l-茶氨酸预处理可能通过抗氧化作用降低镉毒性的严重程度。因此，可以得出结论，l-茶氨酸可用于预防镉诱导的疾病。
l-Theanine is an amino acid derivative from green tea. The present work was aimed at the effect of l-theanine on neuron-like rat pheochromocytoma (PC12) cells stimulated with cadmium chloride. Treatment with l-theanine before cadmium exposure increased cell viability; the experiments of Annexin V/PI staining indicated that l-theanine inhibited cadmium-induced cell apoptosis. Meanwhile, l-theanine decreased ROS production and protected from cadmium-induced disruption of mitochondrial transmembrane potential. Compared with cadmium-treated cells, l-theanine could also decrease the ratio of Bax/Bcl-2, as well as the level of cleaved caspase-9, caspase-3 and poly(ADP-ribose) polymerase. Furthermore, l-theanine depresses cadmium-induced up regulation of phosphorylations of PI3K/Akt, MAPK ERK1/2, and JNK signaling. These data suggest that l-theanine pretreatment reduces severity of cadmium toxicity probably via antioxidant action. Therefore, it may be concluded that l-theanine could be exploited for prevention of cadmium-induced diseases.