Changes in markers of muscle damage, inflammation and HSP70 after an Ironman triathlon race
Changes in markers of muscle damage, inflammation and HSP70 after an Ironman triathlon race
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DOI：
10.1007/s00421-006-0296-4
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发表时间：
2006-12-01
影响因子：
3
通讯作者：
Laursen, Paul B.
中科院分区：
文献类型：
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作者：
Suzuki, Katsuhiko;Peake, Jonathan;Laursen, Paul B.
我们研究了铁人三项赛对肌肉损伤、炎症和热休克蛋白70（HSP 70）标志物的影响。9名训练有素的男性铁人三项运动员（平均+/- SD年龄34 +/- 5岁; VO 2峰值66.4 ml kg（-1）min（-1））参加了2004年西澳大利亚铁人三项比赛（3.8 km游泳，180 km自行车，42.2 km跑步）。我们评估了跳跃高度，肌肉力量和酸痛，并在比赛前2天，比赛后30分钟和14-20小时内采集静脉血样。分析血浆样品的肌肉蛋白、急性期蛋白、细胞因子、热休克蛋白70（HSP 70）和与脱水、溶血、肝和肾功能相关的临床生化变量。运动后肌肉力量和跳跃高度显著下降（P < 0.05），肌肉酸痛和血浆肌肉蛋白浓度升高。白细胞介素（IL）-1受体拮抗剂、IL-6、IL-10和HSP 70在比赛后明显升高，而IL-12 p40和粒细胞集落刺激因子（G-CSF）也升高。IL-4，IL-1 β和肿瘤坏死因子-α没有显着变化，尽管C-反应蛋白和血清淀粉样蛋白A在比赛后一天升高。血浆肌酐、尿酸和总胆红素浓度以及γ-谷氨酰转移酶活性在比赛后也发生了变化。总之，尽管有证据表明，肌肉损伤和急性期反应后的比赛，促炎细胞因子的反应是最小的和抗炎细胞因子的诱导。HSP 70作为运动持续时间的函数被释放到循环中。
We investigated the effects of an Ironman triathlon race on markers of muscle damage, inflammation and heat shock protein 70 (HSP70). Nine well-trained male triathletes (mean +/- SD age 34 +/- 5 years; VO2peak 66.4 ml kg(-1) min(-1)) participated in the 2004 Western Australia Ironman triathlon race (3.8 km swim, 180 km cycle, 42.2 km run). We assessed jump height, muscle strength and soreness, and collected venous blood samples 2 days before the race, within 30 min and 14-20 h after the race. Plasma samples were analysed for muscle proteins, acute phase proteins, cytokines, heat shock protein 70 (HSP70), and clinical biochemical variables related to dehydration, haemolysis, liver and renal functions. Muscular strength and jump height decreased significantly (P < 0.05) after the race, whereas muscle soreness and the plasma concentrations of muscle proteins increased. The cytokines interleukin (IL)-1 receptor antagonist, IL-6 and IL-10, and HSP70 increased markedly after the race, while IL-12p40 and granulocyte colony-stimulating factor (G-CSF) were also elevated. IL-4, IL-1 beta and tumour necrosis factor-alpha did not change significantly, despite elevated C-reactive protein and serum amyloid protein A on the day after the race. Plasma creatinine, uric acid and total bilirubin concentrations and gamma-glutamyl transferase activity also changed after the race. In conclusion, despite evidence of muscle damage and an acute phase response after the race, the pro-inflammatory cytokine response was minimal and anti-inflammatory cytokines were induced. HSP70 is released into the circulation as a function of exercise duration.